ID   VP16_VZVD               Reviewed;         410 AA.
AC   P09265;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   02-JUN-2021, entry version 85.
DE   RecName: Full=Tegument protein VP16 homolog;
DE   AltName: Full=Alpha trans-inducing protein;
DE   AltName: Full=Alpha-TIF;
DE   AltName: Full=ORF10 protein;
DE   AltName: Full=Tegument protein 10;
GN   ORFNames=ORF10;
OS   Varicella-zoster virus (strain Dumas) (HHV-3) (Human herpesvirus 3).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX   NCBI_TaxID=10338;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=3018124; DOI=10.1099/0022-1317-67-9-1759;
RA   Davison A.J., Scott J.E.;
RT   "The complete DNA sequence of varicella-zoster virus.";
RL   J. Gen. Virol. 67:1759-1816(1986).
RN   [2]
RP   FUNCTION, AND INTERACTION WITH HUMAN HCFC1.
RX   PubMed=15522876; DOI=10.1074/jbc.m410178200;
RA   Narayanan A., Nogueira M.L., Ruyechan W.T., Kristie T.M.;
RT   "Combinatorial transcription of herpes simplex virus and varicella zoster
RT   virus immediate early genes is strictly determined by the cellular
RT   coactivator HCF-1.";
RL   J. Biol. Chem. 280:1369-1375(2005).
CC   -!- FUNCTION: Transcriptional activator of immediate-early (IE) gene
CC       products (alpha genes). Acts as a key activator of lytic infection by
CC       initiating the lytic program through the assembly of the
CC       transcriptional regulatory VP16-induced complex composed of VP16 and
CC       two cellular factors, HCFC1 and POU2F1. VP16-induced complex represents
CC       a regulatory switch: when it is on, it promotes IE-gene expression and
CC       thus lytic infection, and when it is off, it limits IE-gene
CC       transcription favoring latent infection (By similarity). {ECO:0000250,
CC       ECO:0000269|PubMed:15522876}.
CC   -!- FUNCTION: May play a role in the aggregation of tegument proteins
CC       around nucleocapsids during virus morphogenesis. {ECO:0000305}.
CC   -!- SUBUNIT: Associates with the VP16-induced complex; binding to host
CC       HCFC1 activates VP16 for association with the octamer motif-binding
CC       host protein POU2F1, to form a multiprotein-DNA complex responsible for
CC       activating transcription of the viral immediate early genes.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P04486}.
CC       Host nucleus {ECO:0000250|UniProtKB:P04486}.
CC   -!- SIMILARITY: Belongs to the herpesviridae tegument protein VP16 protein
CC       family. {ECO:0000305}.
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DR   EMBL; X04370; CAA27893.1; -; Genomic_DNA.
DR   PIR; A27342; IXBE10.
DR   SMR; P09265; -.
DR   PRIDE; P09265; -.
DR   Proteomes; UP000002602; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039695; P:DNA-templated viral transcription; IDA:UniProtKB.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   Gene3D; 1.10.1290.10; -; 1.
DR   InterPro; IPR003174; Alpha_TIF.
DR   InterPro; IPR036538; Alpha_TIF_sf.
DR   Pfam; PF02232; Alpha_TIF; 1.
DR   SMART; SM00814; Alpha_TIF; 1.
DR   SUPFAM; SSF56548; SSF56548; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Host nucleus; Host-virus interaction; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation; Virion;
KW   Virion tegument.
FT   CHAIN           1..410
FT                   /note="Tegument protein VP16 homolog"
FT                   /id="PRO_0000115805"
FT   REGION          388..410
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   410 AA;  46575 MW;  5513A1424325E8F5 CRC64;
     MECNLGTEHP STDTWNRSKT EQAVVDAFDE SLFGDVASDI GFETSLYSHA VKTAPSPPWV
     ASPKILYQQL IRDLDFSEGP RLLSCLETWN EDLFSCFPIN EDLYSDMMVL SPDPDDVIST
     VSTKDHVEMF NLTTRGSVRL PSPPKQPTGL PAYVQEVQDS FTVELRAREE AYTKLLVTYC
     KSIIRYLQGT AKRTTIGLNI QNPDQKAYTQ LRQSILLRYY REVASLARLL YLHLYLTVTR
     EFSWRLYASQ SAHPDVFAAL KFTWTERRQF TCAFHPVLCN HGIVLLEGKP LTASALREIN
     YRRRELGLPL VRCGLVEENK SPLVQQPSFS VHLPRSVGFL THHIKRKLDA YAVKHPQEPR
     HVRADHPYAK VVENRNYGSS IEAMILAPPS PSEILPGDPP RPPTCGFLTR