VP201_ARATH
ID VP201_ARATH Reviewed; 219 AA.
AC Q8GXN6; Q8LEU6; Q9FN01;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 104.
DE RecName: Full=Vacuolar protein sorting-associated protein 20 homolog 1;
DE Short=AtVPS20-1;
DE AltName: Full=Charged multivesicular body protein 6 homolog 1;
DE AltName: Full=ESCRT-III complex subunit VPS20 homolog 1;
GN Name=VPS20.1; Synonyms=CHMP6-1; OrderedLocusNames=At5g63880;
GN ORFNames=MGI19.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=17090720; DOI=10.1242/dev.02654;
RA Spitzer C., Schellmann S., Sabovljevic A., Shahriari M., Keshavaiah C.,
RA Bechtold N., Herzog M., Mueller S., Hanisch F.-G., Huelskamp M.;
RT "The Arabidopsis elch mutant reveals functions of an ESCRT component in
RT cytokinesis.";
RL Development 133:4679-4689(2006).
RN [7]
RP IDENTIFICATION.
RX PubMed=16488176; DOI=10.1016/j.tplants.2006.01.008;
RA Winter V., Hauser M.-T.;
RT "Exploring the ESCRTing machinery in eukaryotes.";
RL Trends Plant Sci. 11:115-123(2006).
RN [8]
RP INTERACTION WITH SKD1.
RC STRAIN=cv. Columbia;
RX PubMed=20663085; DOI=10.1111/j.1365-313x.2010.04310.x;
RA Shahriari M., Keshavaiah C., Scheuring D., Sabovljevic A., Pimpl P.,
RA Haeusler R.E., Huelskamp M., Schellmann S.;
RT "The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of
RT Arabidopsis thaliana.";
RL Plant J. 64:71-85(2010).
RN [9]
RP INTERACTION WITH SKD1, AND REVIEW ON ESCRT-III.
RX PubMed=24812106; DOI=10.1104/pp.114.238378;
RA Cai Y., Zhuang X., Gao C., Wang X., Jiang L.;
RT "The Arabidopsis endosomal sorting complex required for transport III
RT regulates internal vesicle formation of the prevacuolar compartment and is
RT required for plant development.";
RL Plant Physiol. 165:1328-1343(2014).
CC -!- FUNCTION: Component of the ESCRT-III complex, which is required for
CC multivesicular bodies (MVBs) formation and sorting of endosomal cargo
CC proteins into MVBs. The ESCRT-III complex is probably involved in the
CC concentration of MVB cargo (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the endosomal sorting required for transport
CC complex III (ESCRT-III), composed at least of VPS2, VPS20, VPS24 and
CC VPS32 (By similarity). Interacts with SKD1 (PubMed:20663085,
CC PubMed:24812106). {ECO:0000250|UniProtKB:Q96FZ7,
CC ECO:0000269|PubMed:20663085, ECO:0000269|PubMed:24812106}.
CC -!- INTERACTION:
CC Q8GXN6; Q9FY89: VPS20.2; NbExp=4; IntAct=EBI-3865286, EBI-3865360;
CC Q8GXN6; Q8VZC9: VPS25; NbExp=4; IntAct=EBI-3865286, EBI-3865350;
CC Q8GXN6; O82197: VPS32.1; NbExp=4; IntAct=EBI-3865286, EBI-3865938;
CC Q8GXN6; Q9FF81: VPS36; NbExp=5; IntAct=EBI-3865286, EBI-3865302;
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8GXN6-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11036.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB007646; BAB11036.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED97809.1; -; Genomic_DNA.
DR EMBL; BT005575; AAO63995.1; -; mRNA.
DR EMBL; AK118136; BAC42761.1; -; mRNA.
DR EMBL; AY085225; AAM62458.1; -; mRNA.
DR RefSeq; NP_568980.1; NM_125783.4. [Q8GXN6-1]
DR AlphaFoldDB; Q8GXN6; -.
DR SMR; Q8GXN6; -.
DR BioGRID; 21750; 10.
DR IntAct; Q8GXN6; 8.
DR STRING; 3702.AT5G63880.2; -.
DR TCDB; 3.A.31.1.2; the endosomal sorting complexes required for transport iii (escrt-iii) family.
DR ProteomicsDB; 242622; -. [Q8GXN6-1]
DR EnsemblPlants; AT5G63880.1; AT5G63880.1; AT5G63880. [Q8GXN6-1]
DR GeneID; 836508; -.
DR Gramene; AT5G63880.1; AT5G63880.1; AT5G63880. [Q8GXN6-1]
DR KEGG; ath:AT5G63880; -.
DR Araport; AT5G63880; -.
DR eggNOG; KOG2910; Eukaryota.
DR InParanoid; Q8GXN6; -.
DR OMA; GKCELDN; -.
DR PhylomeDB; Q8GXN6; -.
DR PRO; PR:Q8GXN6; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8GXN6; baseline and differential.
DR Genevisible; Q8GXN6; AT.
DR GO; GO:0000815; C:ESCRT III complex; IBA:GO_Central.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR22761; PTHR22761; 1.
DR Pfam; PF03357; Snf7; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Endosome; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..219
FT /note="Vacuolar protein sorting-associated protein 20
FT homolog 1"
FT /id="PRO_0000368202"
FT REGION 171..219
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 20..60
FT /evidence="ECO:0000255"
FT COMPBIAS 172..187
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 140
FT /note="A -> S (in Ref. 5; AAM62458)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 219 AA; 24794 MW; F36422A0975124E4 CRC64;
MGNLFVKKPQ ITEVDRAILS LKTQRRKLGQ YQQKLEKVIE AEKQAARDLI REKRKDRALL
ALRKKRTQEE LLKQVDQWVI NVEQQLTDIE LTSKQKAVFE SLKQGNSAIK AIQSELDLDD
VQKLMDDTAD AKAYQDELNA ILGEKLSAED EEDILAEFDN LESQLIVDEM PEVPTKESEE
SEKLDLPDVP TKTPVASNAE ITPAESATKT KVLEEPLPA
