VP22_BHV1C
ID VP22_BHV1C Reviewed; 258 AA.
AC P30022; Q77CE3;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 72.
DE RecName: Full=Tegument protein VP22;
GN ORFNames=UL49;
OS Bovine herpesvirus 1.1 (strain Cooper) (BoHV-1) (Infectious bovine
OS rhinotracheitis virus).
OC Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC Herpesvirales; Herpesviridae; Alphaherpesvirinae; Varicellovirus.
OX NCBI_TaxID=10323;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9010999; DOI=10.1016/s0378-1135(96)01235-7;
RA Schwyzer M., Styger D., Vogt B., Lowery D.E., Simard C., LaBoissiere S.,
RA Misra V., Vlcek C., Paces V.;
RT "Gene contents in a 31-kb segment at the left genome end of bovine
RT herpesvirus-1.";
RL Vet. Microbiol. 53:67-77(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7745736; DOI=10.1128/jvi.69.6.3863-3867.1995;
RA Liang X., Chow B., Li Y., Raggo C., Yoo D., Attah-Poku S., Babiuk L.A.;
RT "Characterization of bovine herpesvirus 1 UL49 homolog gene and product:
RT bovine herpesvirus 1 UL49 homolog is dispensable for virus growth.";
RL J. Virol. 69:3863-3867(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Schwyzer M., Paces V., Letchworth G.J., Misra V., Buhk H.J., Lowery D.E.,
RA Simard C., Bello L.J., Thiry E., Vlcek C.;
RT "Complete DNA sequence of bovine herpesvirus 1.";
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-95.
RX PubMed=8391186; DOI=10.1006/viro.1993.1344;
RA Liang X., Tang M., Manns B., Babiuk L.A., Zamb T.J.;
RT "Identification and deletion mutagenesis of the bovine herpesvirus 1
RT dUTPase gene and a gene homologous to herpes simplex virus UL49.5.";
RL Virology 195:42-50(1993).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 64-258.
RX PubMed=1327963; DOI=10.1016/0378-1119(92)90280-3;
RA Carpenter D.E., Misra V.;
RT "Sequences of the bovine herpesvirus 1 homologue of herpes simplex virus
RT type-1 alpha-trans-inducing factor (UL48).";
RL Gene 119:259-263(1992).
RN [6]
RP SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, NUCLEAR EXPORT SIGNAL,
RP AND MUTAGENESIS OF 131-PRO--ARG-134.
RC STRAIN=P8-2;
RX PubMed=16140763; DOI=10.1128/jvi.79.18.11864-11872.2005;
RA Zheng C., Brownlie R., Babiuk L.A., van Drunen Littel-van den Hurk S.;
RT "Characterization of the nuclear localization and nuclear export signals of
RT bovine herpesvirus 1 VP22.";
RL J. Virol. 79:11864-11872(2005).
CC -!- FUNCTION: Tegument protein that plays different roles during the time
CC course of infection (By similarity). Participates in both the
CC accumulation of viral mRNAs and viral protein translation at late time
CC of infection (By similarity). Modulates the RNase activity of the
CC virion host shutoff protein UL41 probably to ensure necessary levels of
CC key cellular mRNAs and proteins (By similarity). Plays a role in
CC microtubule reorganization that occurs after viral infection by
CC stabilizing microtubule network (By similarity). Plays a role in the
CC inhibition of host innate immune system by targeting the CGAS enzymatic
CC activity which is the principal cytosolic DNA sensor that detects
CC invading viral DNA. Acts by mediating disruption of liquid-like
CC droplets in which CGAS is activated, thereby preventing CGAS activity
CC (By similarity). {ECO:0000250|UniProtKB:P10233}.
CC -!- SUBUNIT: Interacts with gE (via C-terminus); this interaction is
CC necessary for the recruitment of VP22 to the Golgi and its packaging
CC into virions (By similarity). Interacts with gM (via C-terminus) (By
CC similarity). Interacts with VP16; this interaction allows the formation
CC of a tripartite complex composed of VP16, VP22 and UL41/VHS (By
CC similarity). Interacts with the capsid-binding protein UL16 (By
CC similarity). Interacts with host CGAS (By similarity).
CC {ECO:0000250|UniProtKB:P10233}.
CC -!- INTERACTION:
CC P30022; Q76PF3: US3; Xeno; NbExp=3; IntAct=EBI-11420994, EBI-11420970;
CC -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P10233}.
CC Host cytoplasm {ECO:0000269|PubMed:16140763}. Host nucleus
CC {ECO:0000269|PubMed:16140763}. Host Golgi apparatus
CC {ECO:0000250|UniProtKB:P10233}. Note=One of the most abundant tegument
CC protein (about 2000 copies per virion). Localizes in the cytoplasm at 8
CC hours postinfection and in the nucleus at 16 hours postinfection.
CC During virion morphogenesis, this protein probably accumulates at the
CC trans-Golgi where secondary envelopment occurs.
CC {ECO:0000250|UniProtKB:P10233}.
CC -!- PTM: Highly phosphorylated in the host cell. Packaging is selective for
CC underphosphorylated forms. {ECO:0000250|UniProtKB:P10233}.
CC -!- SIMILARITY: Belongs to the alphaherpesvirinae VP22 tegument protein
CC family. {ECO:0000305}.
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DR EMBL; Z54206; CAA90920.1; -; Genomic_DNA.
DR EMBL; U21137; AAA85715.1; -; Genomic_DNA.
DR EMBL; AJ004801; CAA06085.1; -; Genomic_DNA.
DR EMBL; Z11610; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR PIR; S24228; S24228.
DR RefSeq; NP_045310.1; NC_001847.1.
DR SMR; P30022; -.
DR IntAct; P30022; 2.
DR GeneID; 1487397; -.
DR KEGG; vg:1487397; -.
DR GO; GO:0030430; C:host cell cytoplasm; IMP:AgBase.
DR GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IDA:UniProtKB.
DR GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR GO; GO:0042393; F:histone binding; IMP:AgBase.
DR GO; GO:0039525; P:modulation by virus of host chromatin organization; IMP:GO_Central.
DR InterPro; IPR006908; Herpes_UL49.
DR Pfam; PF04823; Herpes_UL49_2; 1.
PE 1: Evidence at protein level;
KW Host cytoplasm; Host Golgi apparatus; Host nucleus; Phosphoprotein; Virion;
KW Virion tegument.
FT CHAIN 1..258
FT /note="Tegument protein VP22"
FT /id="PRO_0000116094"
FT REGION 66..143
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 234..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 131..134
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000269|PubMed:16140763"
FT MOTIF 204..216
FT /note="Nuclear export signal"
FT /evidence="ECO:0000269|PubMed:16140763"
FT MUTAGEN 131..134
FT /note="PRPR->AAAA: Complete loss of nuclear localization."
FT /evidence="ECO:0000269|PubMed:16140763"
FT MUTAGEN 204..216
FT /note="LDRMLKSAAIRIL->ADRMAKSAAARAA: Complete loss of
FT cytoplasmic localization."
SQ SEQUENCE 258 AA; 26865 MW; 78C5567442E8A161 CRC64;
MARFHRPSED EDDYEYSDLW VRENSLYDYE SGSDDHVYEE LRAATSGPEP SGRRASVRAC
ASAAAVQPAA RGRDRAAAAG TTVAAPAAAP ARRSSSRASS RPPRAAADPP VLRPATRGSS
GGAGAVAVGP PRPRAPPGAN AVASGRPLAF SAAPKTPKAP WCGPTHAYNR TIFCEAVALV
AAEYARQAAA SVWDSDPPKS NERLDRMLKS AAIRILVCEG SGLLAAANDI LAARAQRPAA
RGSTSGGESR LRGERARP
