CALML_DICDI
ID CALML_DICDI Reviewed; 149 AA.
AC O00897; Q55DS1;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Calmodulin-like protein;
GN Name=calB; ORFNames=DDB_G0269104;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RC STRAIN=AX3;
RX PubMed=10818233; DOI=10.1016/s0014-5793(00)01551-9;
RA Rosel D., Puta F., Blahuskova A., Smykal P., Folk P.;
RT "Molecular characterization of a calmodulin-like dictyostelium protein
RT CalB.";
RL FEBS Lett. 473:323-327(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Mediates the control of a large number of enzymes, ion
CC channels and other proteins by Ca(2+) ions. Among the enzymes to be
CC stimulated by the calmodulin-Ca(2+) complex are a number of protein
CC kinases and phosphatases (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Contractile vacuole {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: The calB transcript is very low in vegetative
CC cells, it accumulates to higher levels during aggregation and during
CC slug formation. It is present in its highest level in spores.
CC {ECO:0000269|PubMed:10818233}.
CC -!- SIMILARITY: Belongs to the calmodulin family. {ECO:0000305}.
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DR EMBL; AF001981; AAB60882.1; -; Genomic_DNA.
DR EMBL; AAFI02000005; EAL71901.1; -; Genomic_DNA.
DR RefSeq; XP_646060.1; XM_640968.1.
DR AlphaFoldDB; O00897; -.
DR SMR; O00897; -.
DR STRING; 44689.DDB0191193; -.
DR PaxDb; O00897; -.
DR EnsemblProtists; EAL71901; EAL71901; DDB_G0269104.
DR GeneID; 8617008; -.
DR KEGG; ddi:DDB_G0269104; -.
DR dictyBase; DDB_G0269104; calB.
DR eggNOG; KOG0027; Eukaryota.
DR HOGENOM; CLU_061288_2_0_1; -.
DR InParanoid; O00897; -.
DR OMA; NGYANIQ; -.
DR PhylomeDB; O00897; -.
DR Reactome; R-DDI-111932; CaMK IV-mediated phosphorylation of CREB.
DR Reactome; R-DDI-111957; Cam-PDE 1 activation.
DR Reactome; R-DDI-114608; Platelet degranulation.
DR Reactome; R-DDI-1474151; Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
DR Reactome; R-DDI-163615; PKA activation.
DR Reactome; R-DDI-1855204; Synthesis of IP3 and IP4 in the cytosol.
DR Reactome; R-DDI-203615; eNOS activation.
DR Reactome; R-DDI-2871809; FCERI mediated Ca+2 mobilization.
DR Reactome; R-DDI-4086398; Ca2+ pathway.
DR Reactome; R-DDI-442729; CREB1 phosphorylation through the activation of CaMKII/CaMKK/CaMKIV cascasde.
DR Reactome; R-DDI-5218920; VEGFR2 mediated vascular permeability.
DR Reactome; R-DDI-5607763; CLEC7A (Dectin-1) induces NFAT activation.
DR Reactome; R-DDI-5626467; RHO GTPases activate IQGAPs.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR Reactome; R-DDI-9009391; Extra-nuclear estrogen signaling.
DR Reactome; R-DDI-9619229; Activation of RAC1 downstream of NMDARs.
DR Reactome; R-DDI-9648002; RAS processing.
DR PRO; PR:O00897; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0000331; C:contractile vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IBA:GO_Central.
DR GO; GO:0030234; F:enzyme regulator activity; IBA:GO_Central.
DR CDD; cd00051; EFh; 2.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR Pfam; PF13499; EF-hand_7; 2.
DR SMART; SM00054; EFh; 4.
DR SUPFAM; SSF47473; SSF47473; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 4.
PE 2: Evidence at transcript level;
KW Calcium; Metal-binding; Reference proteome; Repeat; Vacuole.
FT CHAIN 1..149
FT /note="Calmodulin-like protein"
FT /id="PRO_0000327723"
FT DOMAIN 6..41
FT /note="EF-hand 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 42..76
FT /note="EF-hand 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 78..113
FT /note="EF-hand 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT DOMAIN 113..148
FT /note="EF-hand 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 19
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 21
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 23
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 25
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 30
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
SQ SEQUENCE 149 AA; 16827 MW; D7ADD31A83EA422D CRC64;
MAKEDTTQAL YKEAFNLFDK DKDGKITIQE LGIVMRSVGS NPTQQELKDI AKEIDDGSGL
VDFSKFSSLM TRKMKYSDSE ADIKQAFKVF DKKGNGYANI QDLKHTLTSI GEKLTKEEFD
NMLKDAKTVD GQIHVDEFVR VIKSSKSFN
