VP26A_RAT
ID VP26A_RAT Reviewed; 327 AA.
AC Q6AY86;
DT 25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT 13-SEP-2004, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Vacuolar protein sorting-associated protein 26A;
DE AltName: Full=Vesicle protein sorting 26A;
GN Name=Vps26a; Synonyms=Vps26;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-315, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: Acts as component of the retromer cargo-selective complex
CC (CSC). The CSC is believed to be the core functional component of
CC retromer or respective retromer complex variants acting to prevent
CC missorting of selected transmembrane cargo proteins into the lysosomal
CC degradation pathway. The recruitment of the CSC to the endosomal
CC membrane involves RAB7A and SNX3.The SNX-BAR retromer mediates
CC retrograde transport of cargo proteins from endosomes to the trans-
CC Golgi network (TGN) and is involved in endosome-to-plasma membrane
CC transport for cargo protein recycling. The SNX3-retromer mediates the
CC retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR
CC retromer pathway. The SNX27-retromer is believed to be involved in
CC endosome-to-plasma membrane trafficking and recycling of a broad
CC spectrum of cargo proteins. The CSC complex seems to act as recruitment
CC hub for other proteins, such as the WASH complex and TBC1D5. Required
CC for retrograde transport of lysosomal enzyme receptor IGF2R. Required
CC to regulate transcytosis of the polymeric immunoglobulin receptor
CC (pIgR-pIgA). Required for the endosomal localization of WASHC2
CC (indicative for the WASH complex). Required for the endosomal
CC localization of TBC1D5. Mediates retromer cargo recognition of SORL1
CC and is involved in trafficking of SORL1 implicated in sorting and
CC processing of APP. Involved in retromer-independent lysosomal sorting
CC of F2R. Involved in recycling of ADRB2. Acts redundantly with VSP26B in
CC SNX-27 mediated endocytic recycling of SLC2A1/GLUT1. Enhances the
CC affinity of SNX27 for PDZ-binding motifs in cargo proteins (By
CC similarity). {ECO:0000250|UniProtKB:O75436,
CC ECO:0000250|UniProtKB:P40336}.
CC -!- SUBUNIT: Component of the heterotrimeric retromer cargo-selective
CC complex (CSC), also described as vacuolar protein sorting subcomplex
CC (VPS), formed by VPS26 (VPS26A or VPS26B), VPS29 and VPS35. The CSC has
CC a highly elongated structure with VPS26 and VPS29 binding independently
CC at opposite distal ends of VPS35 as central platform. The CSC is
CC believed to associate with variable sorting nexins to form functionally
CC distinct retromer complex variants. The originally described retromer
CC complex (also called SNX-BAR retromer) is a pentamer containing the CSC
CC and a heterodimeric membrane-deforming subcomplex formed between SNX1
CC or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the
CC respective CSC and SNX-BAR subcomplexes associate with low affinity.
CC The CSC associates with SNX3 to form a SNX3-retromer complex. The CSC
CC associates with SNX27, the WASH complex and the SNX-BAR subcomplex to
CC form the SNX27-retromer complex. Interacts with VPS29, VPS35, SNX27,
CC SNX1, SNX2, SNX5, SNX6, SNX3, RAB7A, ECPAS, EHD1, WASHC5, SORL1 (By
CC similarity). {ECO:0000250|UniProtKB:O75436,
CC ECO:0000250|UniProtKB:P40336}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P40336}.
CC Endosome membrane {ECO:0000250|UniProtKB:P40336}; Peripheral membrane
CC protein {ECO:0000250|UniProtKB:P40336}. Early endosome
CC {ECO:0000250|UniProtKB:O75436}. Note=Localizes to tubular profiles
CC adjacent to endosomes. Predominantly found in early not late endosomes.
CC {ECO:0000250|UniProtKB:O75436}.
CC -!- SIMILARITY: Belongs to the VPS26 family. {ECO:0000305}.
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DR EMBL; BC079150; AAH79150.1; -; mRNA.
DR RefSeq; NP_001007741.1; NM_001007740.1.
DR AlphaFoldDB; Q6AY86; -.
DR SMR; Q6AY86; -.
DR BioGRID; 263007; 1.
DR CORUM; Q6AY86; -.
DR IntAct; Q6AY86; 3.
DR STRING; 10116.ENSRNOP00000062170; -.
DR iPTMnet; Q6AY86; -.
DR PhosphoSitePlus; Q6AY86; -.
DR jPOST; Q6AY86; -.
DR PaxDb; Q6AY86; -.
DR PRIDE; Q6AY86; -.
DR DNASU; 361846; -.
DR Ensembl; ENSRNOT00000081916; ENSRNOP00000070391; ENSRNOG00000030683.
DR GeneID; 361846; -.
DR KEGG; rno:361846; -.
DR UCSC; RGD:1359254; rat.
DR CTD; 9559; -.
DR RGD; 1359254; Vps26a.
DR eggNOG; KOG3063; Eukaryota.
DR GeneTree; ENSGT00950000183064; -.
DR HOGENOM; CLU_031077_0_0_1; -.
DR InParanoid; Q6AY86; -.
DR OMA; FKQHGKR; -.
DR OrthoDB; 987411at2759; -.
DR PhylomeDB; Q6AY86; -.
DR Reactome; R-RNO-3238698; WNT ligand biogenesis and trafficking.
DR PRO; PR:Q6AY86; -.
DR Proteomes; UP000002494; Chromosome 20.
DR Bgee; ENSRNOG00000030683; Expressed in testis and 18 other tissues.
DR ExpressionAtlas; Q6AY86; baseline and differential.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005769; C:early endosome; ISS:UniProtKB.
DR GO; GO:0005768; C:endosome; ISS:UniProtKB.
DR GO; GO:0010008; C:endosome membrane; ISO:RGD.
DR GO; GO:0005764; C:lysosome; IEA:Ensembl.
DR GO; GO:0030904; C:retromer complex; IDA:ParkinsonsUK-UCL.
DR GO; GO:0030906; C:retromer, cargo-selective complex; ISO:RGD.
DR GO; GO:0097422; C:tubular endosome; ISS:UniProtKB.
DR GO; GO:0031982; C:vesicle; ISS:UniProtKB.
DR GO; GO:0032456; P:endocytic recycling; ISS:UniProtKB.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; ISS:UniProtKB.
DR Gene3D; 2.60.40.640; -; 2.
DR InterPro; IPR014752; Arrestin-like_C.
DR InterPro; IPR028934; Vps26-related.
DR PANTHER; PTHR12233; PTHR12233; 1.
DR Pfam; PF03643; Vps26; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Endosome; Membrane; Phosphoprotein; Protein transport;
KW Reference proteome; Transport.
FT CHAIN 1..327
FT /note="Vacuolar protein sorting-associated protein 26A"
FT /id="PRO_0000073009"
FT REGION 306..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 312..327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 315
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
SQ SEQUENCE 327 AA; 38115 MW; 88B3B0B48EE6862A CRC64;
MSFLGGFFGP ICEIDVALSD GETRKMAEMK TEDGKVEKHY LFYDGESVSG KVNLAFKQPG
KRLEHQGIRI EFVGQIELFN DKSNTHEFVN LVKELALPGE LTQSRSYDFE FMQVEKPYES
YIGANVRLRY FLKVTIVRRL TDLVKEYDLI VHQLATYPEV NNSIKMEVGI EDCLHIEFEY
NKSKYHLKDV IVGKIYFLLV RIKIQHMELQ LIKKEITGIG PSTTTETETI AKYEIMDGAP
VKGESIPIRL FLAGYDPTPT MRDVNKKFSV RYFLNLVLVD EEDRRYFKQQ EIILWRKAPE
KLRKQRTNFH QRFESPESQA SAEQPEM
