ID   VP2_BTV1S               Reviewed;         961 AA.
AC   P32508;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   23-FEB-2022, entry version 63.
DE   RecName: Full=Outer capsid protein VP2;
GN   Name=Segment-2;
OS   Bluetongue virus 1 (isolate South Africa) (BTV 1).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Orbivirus.
OX   NCBI_TaxID=10905;
OH   NCBI_TaxID=9891; Antilocapra americana (Pronghorn).
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=46212; Culicoides variipennis (Biting midge).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 322-346 AND 492-503.
RX   PubMed=2160764; DOI=10.1016/0168-1702(90)90029-b;
RA   Wade-Evans A.M., Mertens P.P.C.;
RT   "Expression of the outer capsid protein, VP2, from a full length cDNA clone
RT   of genome segment 2 of bluetongue serotype 1 from South Africa, using both
RT   Sp6 and vaccinia expression systems and a comparison of the nucleic acid
RT   sequence of this segment with those of other serotypes.";
RL   Virus Res. 15:213-230(1990).
RN   [2]
RP   FUNCTION.
RX   PubMed=20332209; DOI=10.1073/pnas.0913403107;
RA   Zhang X., Boyce M., Bhattacharya B., Zhang X., Schein S., Roy P.,
RA   Zhou Z.H.;
RT   "Bluetongue virus coat protein VP2 contains sialic acid-binding domains,
RT   and VP5 resembles enveloped virus fusion proteins.";
RL   Proc. Natl. Acad. Sci. U.S.A. 107:6292-6297(2010).
RN   [3]
RP   FUNCTION.
RX   PubMed=17267479; DOI=10.1128/jvi.02284-06;
RA   Forzan M., Marsh M., Roy P.;
RT   "Bluetongue virus entry into cells.";
RL   J. Virol. 81:4819-4827(2007).
CC   -!- FUNCTION: The VP2 protein is one of the two proteins (with VP5) target
CC       of the host immunogenic response. Responsible for viral which
CC       constitute the virus particle outer capsid. It is the major attachment
CC       to target host cell, probably by binding to sialic acid. This
CC       attachment induces virion internalization predominantly through
CC       clathrin-dependent endocytosis. {ECO:0000269|PubMed:17267479,
CC       ECO:0000269|PubMed:20332209}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orbivirus VP2 family. {ECO:0000305}.
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DR   PIR; A60014; P2XRSA.
DR   SMR; P32508; -.
DR   DIP; DIP-59518N; -.
DR   IntAct; P32508; 3.
DR   GO; GO:0039625; C:viral inner capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0075512; P:clathrin-dependent endocytosis of virus by host cell; IEA:UniProtKB-KW.
DR   GO; GO:0019062; P:virion attachment to host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR001742; Capsid_VP2_Orbivir.
DR   Pfam; PF00898; Orbi_VP2; 1.
PE   1: Evidence at protein level;
KW   Capsid protein; Clathrin-mediated endocytosis of virus by host;
KW   Direct protein sequencing; Host-virus interaction; Inner capsid protein;
KW   Viral attachment to host cell; Viral penetration into host cytoplasm;
KW   Virion; Virus endocytosis by host; Virus entry into host cell.
FT   CHAIN           1..961
FT                   /note="Outer capsid protein VP2"
FT                   /id="PRO_0000222681"
SQ   SEQUENCE   961 AA;  111937 MW;  CB7853598EFD197A CRC64;
     MDELGIPVYK RGFPEHLLRG YEFIIDVGTK IESVGGRHDV TKIPEMNAYD IKQESIRTAL
     WYNPIRNDGF VLPRVLDITL RGYDERRAVV ESTRHKSFHT NDQWVQWMMK DSMDAQPLKV
     GLDTQVWNVA HSLHNSVVEI DSKKADTMAY HVEPIEDASK GCLHTRTMMW NHLVRIETFH
     AAQEVHILFK PTYDIVVHAE RRDRSQPFRP GDQTLINFGR GQKVAMNHNS YDKMVEGLTH
     LVIRGKTPEV IRDDIASLDE ICNRWIQSRH DPGEIKAYEL CKYLSTIGRK SLDREKEPED
     EANLSIRFQE AIDNKFRQHD PERLKIFEHR NQRRDEDRFY ILLMIAGSDT FNTRVWWSNP
     YPCLRRKLIA SETKLGDVYS MMRSWYDWSV RPTYAPYEKT REQEKYIYGR VNLFDFVAEP
     GIKIIHWEYK LNHSTREITY AQGNPCDYYP EDDDVIVTKF DDVAYGQMIN EMINGGWNQE
     QFKMHKILKS EGNVLTIDFE KDAKLTTNEG VTMPEYFNKW IIAPMFNAKL RIKHEEIAQR
     QSDDPMVKRT LSPIAADPIV LQRLTLARFY DIRPALIGQG LSRQQAQSTY DEEISKQAGY
     AEILKRRGIV QIPKKPCPTV TAQYTLELYS LSLINILQQH VARDCDEEAI YEHPKADYEL
     EIFGESIVDI SQVIVLVFDL IFERRRRVRD VYESRYIITR IRRMRGKERL NVIAEFFPTY
     GSLLNGLNSA YVVQDIMYLN FLPLYFLAGD NMIYSHRQWS IPLLLYTHEV MVIPLEVGSY
     NDRCGLIAYL EYMVFFPSKA IRLSKLNEAH AKIAREMLKY YANTTVYDGG DNSNVVTTKQ
     LLYETYLASL CGGFLDGIVW YLPITHPKKC IVAIEVSDER VPASIRAGRI RLRFPLSSRH
     LKGSAIIQID LVGRFTVYSE GIVSFLVCKK NLLKYKCEII LLKFSGHVFG NDEMLTKLLN
     V