VP2_ROTGI
ID VP2_ROTGI Reviewed; 934 AA.
AC Q86108;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 02-DEC-2020, entry version 45.
DE RecName: Full=Inner capsid protein VP2 {ECO:0000255|HAMAP-Rule:MF_04123};
OS Rotavirus B (isolate RVB/Rat/United States/IDIR/1984/G1P[X]) (RV-B)
OS (Rotavirus B (isolate infectious diarrhea of infant rats)).
OC Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC Reovirales; Reoviridae; Sedoreovirinae; Rotavirus.
OX NCBI_TaxID=28877;
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=10116; Rattus norvegicus (Rat).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=8116237; DOI=10.1006/viro.1994.1106;
RA Lindsay D.A., Waggie K.S., Eiden J.J.;
RT "Group B rotavirus VP2: sequence analysis, expression, and gene coding
RT assignment.";
RL Virology 199:141-150(1994).
CC -!- FUNCTION: Inner capsid protein that self-assembles to form an
CC icosahedral capsid with a T=2 symmetry, which consists of 120 copies of
CC VP2, with channels at each of its five-fold vertices. This capsid
CC constitutes the innermost concentric layer of the viral mature
CC particle. It encapsidates the polymerase VP1, the capping enzyme VP3
CC and the genomic dsRNA, thereby defining the core. The innermost VP2
CC capsid and the intermediate VP6 capsid remain intact following cell
CC entry to protect the dsRNA from degradation and to prevent unfavorable
CC antiviral responses in the host cell during all the replication cycle
CC of the virus. Nascent transcripts are transcribed within the structural
CC confines of this double-layered particle (DLP) and are extruded through
CC the channels formed by VP2 N-termini. VP2 is required for the replicase
CC activity of VP1 polymerase. Probably recruits a copy of a VP1-VP3
CC complex, potentially along with a segment of plus-strand RNA, as a
CC decamer of VP2 assembles. May activate the autoinhibited VP1/RNA
CC complex to coordinate packaging and genome replication.
CC {ECO:0000255|HAMAP-Rule:MF_04123}.
CC -!- SUBUNIT: Homodecamer; each decamer is made up of two conformers of VP2,
CC called VP2A and VP2B. Interacts with a VP1-VP3 complex. Interacts with
CC the intermediate capsid protein VP6. Interacts with NSP5. Interacts
CC (via N-terminus) with NSP2. {ECO:0000255|HAMAP-Rule:MF_04123}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000255|HAMAP-Rule:MF_04123}.
CC Note=Inner capsid protein. Also found in spherical cytoplasmic
CC structures, called virus factories, that appear early after infection
CC and are the site of viral replication and packaging.
CC {ECO:0000255|HAMAP-Rule:MF_04123}.
CC -!- SIMILARITY: Belongs to the rotavirus VP2 family. {ECO:0000255|HAMAP-
CC Rule:MF_04123}.
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DR EMBL; U00673; AAA17401.1; -; Genomic_RNA.
DR PIR; A49808; A49808.
DR GO; GO:0039616; C:T=2 icosahedral viral capsid; IEA:UniProtKB-UniRule.
DR GO; GO:0039625; C:viral inner capsid; IEA:UniProtKB-UniRule.
DR GO; GO:0019013; C:viral nucleocapsid; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR HAMAP; MF_04123; Rota_VP2; 1.
DR InterPro; IPR007779; Rotavirus_VP2.
PE 3: Inferred from homology;
KW Capsid protein; Inner capsid protein; RNA-binding;
KW T=2 icosahedral capsid protein; Virion.
FT CHAIN 1..934
FT /note="Inner capsid protein VP2"
FT /id="PRO_0000369833"
SQ SEQUENCE 934 AA; 106015 MW; F61A623E0B4CA090 CRC64;
MDFPNLVLSA KNSIQNVDSK DEKQKIIDQL ISDIRGQNDG VIPDEVLSEI QQVAEINGLS
FNYEKPVKQE LLEQPDPTSV LSQEVFQIRT ILSKTLFVDV EAEDYSVYIP DETSNLSPVQ
IDARPIQTYQ PKALMYKDTA ILPSNRDEVS DQYGADEILF DSHMFNDISQ AQIRDFETYV
LDKAMQIQSS LPNLDFVSSL DKEVNPFNVH NTLCLNFGQR EYYNIIADRT NQSFQQRRQS
VQFDNVVVDG VARRARVSLR LHPFDSQLLD IIRFNTIQDQ PLADTMAEYQ LVAADGFVAT
PRFRTDRDAR LIADVRSRVM ARLCELSPYF HRTRILSSMT DFNSLWKVNV FSSSIDNAKD
AIYRMAEISF TVADATTSAL SSVNIASAQQ TLLVLLNMSL FRFEIEPVGS QSNFGAAVSA
ALMLVVFPTD EASMSNVTFD NLCNLVFNEL IAWTVDRPTF VKRTGMTNAF EANVNIGGGN
MTRDITAYMR FVLLRRPWAV FQRTYDDQYV ADIMVPNIDE ANVNDQSYMA INNLFSGLIQ
AAQRNPNPGR QIAATSFRKL LKSMKDSCCN RIMPLIRLMK YNVERIARIY RFFPYTADLV
QRIPAFRDER IRIKVPVSGM LSIALGINKS PDVFDWYNLL RFADVIRTKN FAERQSLESI
MVQALIRNDI NPARSRKEYI QQNIKPATNV VASITKVPSA TFTTILSDRM LNNEIRRTQS
YIVVNRIRDA VRAAFEHVPT AEHGIAKGAL LLPYPQNFQR SSVYVRKDNI LYNAPVGVDR
FSLDDLLNGR FYQGMVNRIQ NMSPFVIAGP LQVRASNASA IDSVTSAYLT MSSPYDACVK
PEDLRHNRIV QPPIVDFFSD SSITRPNTQF EQLMSKTSVF VIDAPKLIVQ SDATVYNFDY
RDIQLTTSVV DKLEFTSVKT PDVTLFNGML VFED