VP321_ARATH
ID VP321_ARATH Reviewed; 213 AA.
AC O82197;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Vacuolar protein sorting-associated protein 32 homolog 1;
DE Short=AtVPS32-1;
DE AltName: Full=Charged multivesicular body protein 4 homolog 1;
DE AltName: Full=ESCRT-III complex subunit VPS32 homolog 1;
GN Name=VPS32.1; Synonyms=CHMP4-1, SNF7A {ECO:0000303|PubMed:22639582};
GN OrderedLocusNames=At2g19830; ORFNames=F6F22.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION, AND NOMENCLATURE.
RX PubMed=17090720; DOI=10.1242/dev.02654;
RA Spitzer C., Schellmann S., Sabovljevic A., Shahriari M., Keshavaiah C.,
RA Bechtold N., Herzog M., Mueller S., Hanisch F.-G., Huelskamp M.;
RT "The Arabidopsis elch mutant reveals functions of an ESCRT component in
RT cytokinesis.";
RL Development 133:4679-4689(2006).
RN [5]
RP IDENTIFICATION.
RX PubMed=16488176; DOI=10.1016/j.tplants.2006.01.008;
RA Winter V., Hauser M.-T.;
RT "Exploring the ESCRTing machinery in eukaryotes.";
RL Trends Plant Sci. 11:115-123(2006).
RN [6]
RP INTERACTION WITH SKD1.
RC STRAIN=cv. Columbia;
RX PubMed=20663085; DOI=10.1111/j.1365-313x.2010.04310.x;
RA Shahriari M., Keshavaiah C., Scheuring D., Sabovljevic A., Pimpl P.,
RA Haeusler R.E., Huelskamp M., Schellmann S.;
RT "The AAA-type ATPase AtSKD1 contributes to vacuolar maintenance of
RT Arabidopsis thaliana.";
RL Plant J. 64:71-85(2010).
RN [7]
RP NOMENCLATURE, AND INTERACTION WITH BRO1.
RX PubMed=22639582; DOI=10.3389/fpls.2011.00020;
RA Richardson L.G., Howard A.S., Khuu N., Gidda S.K., McCartney A.,
RA Morphy B.J., Mullen R.T.;
RT "Protein-protein interaction network and subcellular localization of the
RT Arabidopsis thaliana ESCRT machinery.";
RL Front. Plant Sci. 2:20-20(2011).
RN [8]
RP INTERACTION WITH BRO1.
RX PubMed=26342016; DOI=10.1105/tpc.15.00393;
RA Cardona-Lopez X., Cuyas L., Marin E., Rajulu C., Irigoyen M.L., Gil E.,
RA Puga M.I., Bligny R., Nussaume L., Geldner N., Paz-Ares J., Rubio V.;
RT "ESCRT-III-associated protein ALIX mediates high-affinity phosphate
RT transporter trafficking to maintain phosphate homeostasis in Arabidopsis.";
RL Plant Cell 27:2560-2581(2015).
CC -!- FUNCTION: Component of the ESCRT-III complex, which is required for
CC multivesicular bodies (MVBs) formation and sorting of endosomal cargo
CC proteins into MVBs. The ESCRT-III complex is probably involved in the
CC concentration of MVB cargo (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the endosomal sorting required for transport
CC complex III (ESCRT-III), composed at least of VPS2, VPS20, VPS24 and
CC VPS32 (By similarity). Interacts with SKD1 (PubMed:20663085). Interacts
CC with BRO1/ALIX (PubMed:22639582, PubMed:26342016).
CC {ECO:0000250|UniProtKB:Q9H444, ECO:0000269|PubMed:20663085,
CC ECO:0000269|PubMed:22639582, ECO:0000269|PubMed:26342016}.
CC -!- INTERACTION:
CC O82197; Q8GXN6: VPS20.1; NbExp=4; IntAct=EBI-3865938, EBI-3865286;
CC O82197; Q9FY89: VPS20.2; NbExp=3; IntAct=EBI-3865938, EBI-3865360;
CC O82197; Q9SZE4: VPS32.2; NbExp=4; IntAct=EBI-3865938, EBI-3865953;
CC -!- SUBCELLULAR LOCATION: Endosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the SNF7 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC005169; AAC62133.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61252.1; -; Genomic_DNA.
DR EMBL; AY045911; AAK76585.1; -; mRNA.
DR EMBL; AY079421; AAL85152.1; -; mRNA.
DR PIR; F84581; F84581.
DR RefSeq; NP_179573.1; NM_127541.5.
DR AlphaFoldDB; O82197; -.
DR SMR; O82197; -.
DR BioGRID; 1857; 11.
DR IntAct; O82197; 6.
DR STRING; 3702.AT2G19830.1; -.
DR TCDB; 3.A.31.1.2; the endosomal sorting complexes required for transport iii (escrt-iii) family.
DR MetOSite; O82197; -.
DR PaxDb; O82197; -.
DR PRIDE; O82197; -.
DR ProteomicsDB; 242625; -.
DR EnsemblPlants; AT2G19830.2; AT2G19830.2; AT2G19830.
DR GeneID; 816502; -.
DR Gramene; AT2G19830.2; AT2G19830.2; AT2G19830.
DR KEGG; ath:AT2G19830; -.
DR Araport; AT2G19830; -.
DR TAIR; locus:2051970; AT2G19830.
DR eggNOG; KOG1656; Eukaryota.
DR HOGENOM; CLU_071097_2_0_1; -.
DR InParanoid; O82197; -.
DR OMA; EHKIQQE; -.
DR OrthoDB; 1490465at2759; -.
DR PhylomeDB; O82197; -.
DR PRO; PR:O82197; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O82197; baseline and differential.
DR Genevisible; O82197; AT.
DR GO; GO:0009898; C:cytoplasmic side of plasma membrane; IBA:GO_Central.
DR GO; GO:0000815; C:ESCRT III complex; ISS:TAIR.
DR GO; GO:0005771; C:multivesicular body; IBA:GO_Central.
DR GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0006900; P:vesicle budding from membrane; IBA:GO_Central.
DR InterPro; IPR005024; Snf7_fam.
DR PANTHER; PTHR22761; PTHR22761; 1.
DR Pfam; PF03357; Snf7; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Protein transport; Reference proteome; Transport.
FT CHAIN 1..213
FT /note="Vacuolar protein sorting-associated protein 32
FT homolog 1"
FT /id="PRO_0000368200"
FT REGION 180..213
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 11..42
FT /evidence="ECO:0000255"
FT COILED 118..176
FT /evidence="ECO:0000255"
SQ SEQUENCE 213 AA; 24014 MW; FB7EBA169C56F1EC CRC64;
MFMNRLFGKP KQETSTLQTL DKLNETLEML EKKENVLLKK ATGEVEKAKE FSRAKNKRAA
IQCLKRKRLY EQQVEQLGNF QLRIHDQMIM LEGAKATTET VDALRTGASA MKAMQKATNI
DDVDKTMDEI NEQTENMKQI QEALSAPFGA NDFDEDELEA ELDELEGAEL EEQLLQPVPI
HVPQGNKPAR APAQKQPTAE EDELAALQAE MAL
