ID   VP32_BPPRD              Reviewed;          54 AA.
AC   Q3T4M7;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2005, sequence version 1.
DT   07-OCT-2020, entry version 60.
DE   RecName: Full=Protein P32;
GN   Name=XXXII;
OS   Enterobacteria phage PRD1 (Bacteriophage PRD1).
OC   Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes;
OC   Kalamavirales; Tectiviridae; Alphatectivirus.
OX   NCBI_TaxID=10658;
OH   NCBI_TaxID=471; Acinetobacter calcoaceticus.
OH   NCBI_TaxID=562; Escherichia coli.
OH   NCBI_TaxID=584; Proteus mirabilis.
OH   NCBI_TaxID=287; Pseudomonas aeruginosa.
OH   NCBI_TaxID=294; Pseudomonas fluorescens.
OH   NCBI_TaxID=303; Pseudomonas putida (Arthrobacter siderocapsulatus).
OH   NCBI_TaxID=90371; Salmonella typhimurium.
OH   NCBI_TaxID=666; Vibrio cholerae.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1853567; DOI=10.1016/0042-6822(91)90995-n;
RA   Bamford J.K.H., Haenninen A.-L., Pakula T.M., Ojala P.M., Kalkkinen N.,
RA   Frilander M., Bamford D.H.;
RT   "Genome organization of membrane-containing bacteriophage PRD1.";
RL   Virology 183:658-676(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=15946683; DOI=10.1016/j.jmb.2005.04.059;
RA   Saren A.M., Ravantti J.J., Benson S.D., Burnett R.M., Paulin L.,
RA   Bamford D.H., Bamford J.K.H.;
RT   "A snapshot of viral evolution from genome analysis of the tectiviridae
RT   family.";
RL   J. Mol. Biol. 350:427-440(2005).
RN   [3]
RP   FUNCTION.
RX   PubMed=12453208; DOI=10.1046/j.1365-2958.2002.03250.x;
RA   Grahn A.M., Daugelavicius R., Bamford D.H.;
RT   "Sequential model of phage PRD1 DNA delivery: active involvement of the
RT   viral membrane.";
RL   Mol. Microbiol. 46:1199-1209(2002).
RN   [4]
RP   FUNCTION.
RX   PubMed=11967303; DOI=10.1128/jvi.76.10.4866-4872.2002;
RA   Grahn A.M., Daugelavicius R., Bamford D.H.;
RT   "The small viral membrane-associated protein P32 is involved in
RT   bacteriophage PRD1 DNA entry.";
RL   J. Virol. 76:4866-4872(2002).
CC   -!- FUNCTION: Component of the phage injection machinery. Required for DNA
CC       injection in the membrane transformation event. Involved in the
CC       formation of the membrane tail tube to connect the virus interior with
CC       the host cytosol. Essential for viral infectivity.
CC       {ECO:0000269|PubMed:11967303, ECO:0000269|PubMed:12453208}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000305}. Note=Part of the capsid inner membrane.
CC       {ECO:0000305}.
CC   -!- MISCELLANEOUS: PRD1 virions are composed of a tail-less icosahedral
CC       capsid of diameter 63 nm, an inner protein-lipid membrane, and a dsDNA
CC       genome which is located inside the lipid vesicle. The DNA is packaged
CC       into a preformed procapsid. The internal membrane plays an active role
CC       in DNA delivery to the host cell by forming a tubular structure used
CC       for injecting the DNA into the host cytoplasm.
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DR   EMBL; AY848689; AAX45919.1; -; Genomic_DNA.
DR   RefSeq; YP_001542612.1; NC_001421.2.
DR   SMR; Q3T4M7; -.
DR   TCDB; 1.K.2.1.1; the prd1 phage dna delivery (prd1-dd) family.
DR   Proteomes; UP000002143; Genome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039641; C:viral inner membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR   InterPro; IPR020085; DNA_delivery_prot_P32/34.
DR   Pfam; PF11087; PRD1_DD; 1.
PE   4: Predicted;
KW   Capsid inner membrane protein; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix; Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Virion; Virus entry into host cell.
FT   CHAIN           1..54
FT                   /note="Protein P32"
FT                   /id="PRO_0000234090"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   54 AA;  5457 MW;  A640CC34F11B8A55 CRC64;
     MGEFGKTLIT IVTAIIGVAI IAVIVSQRSN TAGVIQSATS GFSNILKSAL APII