ID   VP35B_ARATH             Reviewed;         790 AA.
AC   F4I0P8; Q0WRZ0; Q9LQS9;
DT   14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Vacuolar protein sorting-associated protein 35B;
DE   AltName: Full=Vesicle protein sorting 35B;
GN   Name=VPS35B; OrderedLocusNames=At1g75850; ORFNames=T4O12.9;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   COMPONENT OF THE RETROMER COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=16582012; DOI=10.1105/tpc.105.035907;
RA   Oliviusson P., Heinzerling O., Hillmer S., Hinz G., Tse Y.C., Jiang L.,
RA   Robinson D.G.;
RT   "Plant retromer, localized to the prevacuolar compartment and microvesicles
RT   in Arabidopsis, may interact with vacuolar sorting receptors.";
RL   Plant Cell 18:1239-1252(2006).
RN   [5]
RP   TISSUE SPECIFICITY, FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=18222962; DOI=10.1093/pcp/pcn006;
RA   Yamazaki M., Shimada T., Takahashi H., Tamura K., Kondo M., Nishimura M.,
RA   Hara-Nishimura I.;
RT   "Arabidopsis VPS35, a retromer component, is required for vacuolar protein
RT   sorting and involved in plant growth and leaf senescence.";
RL   Plant Cell Physiol. 49:142-156(2008).
CC   -!- FUNCTION: Plays a role in vesicular protein sorting. Component of the
CC       membrane-associated retromer complex which is essential in endosome-to-
CC       Golgi retrograde transport. Also involved in the efficient sorting of
CC       seed storage proteins globulin 12S and albumin 2S. The VPS29-VPS26-
CC       VPS35 subcomplex may be involved in recycling of specific cargos from
CC       endosome to the plasma membrane. {ECO:0000269|PubMed:18222962}.
CC   -!- SUBUNIT: Component of the retromer complex which consists of VPS29
CC       (MAG1), VPS26 (VPS26A or VPS26B), VPS35 (VPS35A or VPS35B or VPS35C),
CC       VPS5/17 (SNX1 or SNX2A or SNX2B). Component of a retromer subcomplex
CC       consisting of VPS29 (MAG1), VPS26 (VPS26A or VPS26B), VPS35 (VPS35A or
CC       VPS35B or VPS35C).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Endosome membrane; Peripheral membrane
CC       protein; Cytoplasmic side. Prevacuolar compartment membrane; Peripheral
CC       membrane protein; Cytoplasmic side. Golgi apparatus, trans-Golgi
CC       network membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}; Cytoplasmic side {ECO:0000250}.
CC   -!- TISSUE SPECIFICITY: Expressed in siliques and maturing seeds (at
CC       protein level). {ECO:0000269|PubMed:18222962}.
CC   -!- SIMILARITY: Belongs to the VPS35 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF26771.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC007396; AAF26771.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE35765.1; -; Genomic_DNA.
DR   EMBL; AK228153; BAF00109.1; -; mRNA.
DR   PIR; G96787; G96787.
DR   RefSeq; NP_177713.3; NM_106235.5.
DR   AlphaFoldDB; F4I0P8; -.
DR   SMR; F4I0P8; -.
DR   BioGRID; 29137; 4.
DR   IntAct; F4I0P8; 1.
DR   STRING; 3702.AT1G75850.1; -.
DR   PaxDb; F4I0P8; -.
DR   PRIDE; F4I0P8; -.
DR   ProteomicsDB; 242702; -.
DR   EnsemblPlants; AT1G75850.1; AT1G75850.1; AT1G75850.
DR   GeneID; 843918; -.
DR   Gramene; AT1G75850.1; AT1G75850.1; AT1G75850.
DR   KEGG; ath:AT1G75850; -.
DR   Araport; AT1G75850; -.
DR   TAIR; locus:2204390; AT1G75850.
DR   eggNOG; KOG1107; Eukaryota.
DR   HOGENOM; CLU_005836_1_0_1; -.
DR   InParanoid; F4I0P8; -.
DR   OMA; INTEMQG; -.
DR   OrthoDB; 316875at2759; -.
DR   PRO; PR:F4I0P8; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I0P8; baseline and differential.
DR   Genevisible; F4I0P8; AT.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005770; C:late endosome; IBA:GO_Central.
DR   GO; GO:0030904; C:retromer complex; IBA:GO_Central.
DR   GO; GO:0030906; C:retromer, cargo-selective complex; IEA:InterPro.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR   Gene3D; 1.25.40.660; -; 1.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR005378; Vps35.
DR   InterPro; IPR042491; Vps35_C.
DR   PANTHER; PTHR11099; PTHR11099; 1.
DR   Pfam; PF03635; Vps35; 1.
DR   PIRSF; PIRSF009375; Retromer_Vps35; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Endosome; Golgi apparatus; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..790
FT                   /note="Vacuolar protein sorting-associated protein 35B"
FT                   /id="PRO_0000414725"
FT   CONFLICT        293
FT                   /note="K -> R (in Ref. 3; BAF00109)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   790 AA;  89445 MW;  F1C9F282C120AD20 CRC64;
     MRTLAGVEDE DKWLAEGIAG IQHNAFFMHR ALDANNLREV LKYSALMLSE LRTSKLSPQK
     YYDLYMRAFD QLRQLEIFFK DESRHGLPVV DLYELVQHAG NILPRMYLLC TVGSVYIKSK
     QAPSKDVLKD LVEMCRGVQH PIRGLFLRSY LAQVSRDKLP EIGSDYEGDA NTVMDAVEFV
     LQNFTEMNKL WVRIQHQGPG TVREKQEKER NELRDLVGKN LHVLGQIEGV DLEMYKETVL
     PRVLEQVVNC KDKLAQYYLM ECIIQVFPDE YHLQTLETLL AACTQLMPTV DTKIVLTQLM
     DRLSNYAASS PDVLHEFLQV EAFAKLSNAI GKVIDTQLEM PIVGAMTLFV SLLTFTLRVH
     PDRLDYVDQV LGACVVKLSS VPKLEDARAM KQVVALLSAP LEKYSDIVTA LTLSNYPRVM
     DHLDDGTNKV MAMLIIQSIM KTDSCISTAD KVEVLFELIK GLIKDLDETN AEELDEEDFQ
     EEQNSVARLI HMLDNEEPEE MLKIICVVRR HLMTGGPRRL PFTVPPLVFS AVRLVRQLES
     QGGDIAGEDS ATPRKIFQIL NQTIEVLTSV PCPELALRLY LQCAEAASDC DLEPVAYEFF
     TQAFMLYEEE IADSKAQVTA IHLIVGTLQR INVFGVENRD TLTHKATGYS ARLLKKPDQC
     RAVYACSHLF WVDDPDGIKD GERVLLCLRR ALRIANAAQQ MASATRGSSG PVTLFVEILN
     KYIYFFEKGN PHITPSDIQS LIELINNEMQ SDNGNTTIHS DPFFTSTLRY IKFIKQKGGL
     MGEKYDPIKL