VP35_MABVM
ID VP35_MABVM Reviewed; 329 AA.
AC P35259; Q38L44; Q6T6U2;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 02-JUN-2021, entry version 73.
DE RecName: Full=Polymerase cofactor VP35;
DE AltName: Full=Marburg VP35 {ECO:0000303|PubMed:30044983};
DE Short=mVP35 {ECO:0000303|PubMed:30044983};
GN Name=VP35;
OS Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain
OS Kenya/Musoke/1980)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina;
OC Monjiviricetes; Mononegavirales; Filoviridae; Marburgvirus.
OX NCBI_TaxID=33727;
OH NCBI_TaxID=9534; Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops).
OH NCBI_TaxID=9606; Homo sapiens (Human).
OH NCBI_TaxID=9407; Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat).
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1626422; DOI=10.1016/0168-1702(92)90027-7;
RA Feldmann H., Muehlberger E., Randolf A., Will C., Kiley M.P., Sanchez A.,
RA Klenk H.-D.;
RT "Marburg virus, a filovirus: messenger RNAs, gene order, and regulatory
RT elements of the replication cycle.";
RL Virus Res. 24:1-19(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=pp3/guinea pig lethal, and pp4/guinea pig nonlethal;
RA Chain P.S.G., Malfatti S.A., Hajjaj A., Vergez L.M., Do L.H., Smith K.L.,
RA McCready P.M.;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=pp3/guinea pig lethal, and pp4/guinea pig nonlethal;
RA Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M.,
RA Schmaljohn A.;
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Enterlein;
RX PubMed=16379005; DOI=10.1128/jvi.80.2.1038-1043.2006;
RA Enterlein S., Volchkov V., Weik M., Kolesnikova L., Volchkova V.,
RA Klenk H.-D., Muehlberger E.;
RT "Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid
RT protein VP30.";
RL J. Virol. 80:1038-1043(2006).
RN [5]
RP SUBUNIT, COILED-COIL DOMAIN, MUTAGENESIS OF LEU-90 AND LEU-104, AND
RP FUNCTION.
RX PubMed=16282487; DOI=10.1128/jvi.79.23.14876-14886.2005;
RA Moeller P., Pariente N., Klenk H.-D., Becker S.;
RT "Homo-oligomerization of Marburgvirus VP35 is essential for its function in
RT replication and transcription.";
RL J. Virol. 79:14876-14886(2005).
RN [6]
RP INTERACTION WITH THE NUCLEOPROTEIN, AND COILED-COIL DOMAIN.
RX PubMed=17958906; DOI=10.1186/1743-422x-4-105;
RA Dicarlo A., Moeller P., Lander A., Kolesnikova L., Becker S.;
RT "Nucleocapsid formation and RNA synthesis of Marburg virus is dependent on
RT two coiled coil motifs in the nucleoprotein.";
RL Virol. J. 4:105-105(2007).
RN [7]
RP FUNCTION, AND NOMENCLATURE.
RX PubMed=30044983; DOI=10.1016/j.celrep.2018.06.045;
RA Edwards M.R., Liu H., Shabman R.S., Ginell G.M., Luthra P., Ramanan P.,
RA Keefe L.J., Koellner B., Amarasinghe G.K., Taylor D.J., Leung D.W.,
RA Basler C.F.;
RT "Conservation of Structure and Immune Antagonist Functions of Filoviral
RT VP35 Homologs Present in Microbat Genomes.";
RL Cell Rep. 24:861-872(2018).
RN [8] {ECO:0007744|PDB:4GH9, ECO:0007744|PDB:4GHA}
RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 204-329, AND FUNCTION.
RX PubMed=23028316; DOI=10.1371/journal.ppat.1002916;
RA Bale S., Julien J.P., Bornholdt Z.A., Kimberlin C.R., Halfmann P.,
RA Zandonatti M.A., Kunert J., Kroon G.J., Kawaoka Y., MacRae I.J.,
RA Wilson I.A., Saphire E.O.;
RT "Marburg virus VP35 can both fully coat the backbone and cap the ends of
RT dsRNA for interferon antagonism.";
RL PLoS Pathog. 8:e1002916-e1002916(2012).
RN [9] {ECO:0007744|PDB:5TOH, ECO:0007744|PDB:5TOI}
RP X-RAY CRYSTALLOGRAPHY (2.01 ANGSTROMS) OF 60-130.
RX PubMed=27847355; DOI=10.1128/jvi.01085-16;
RA Bruhn J.F., Kirchdoerfer R.N., Urata S.M., Li S., Tickle I.J., Bricogne G.,
RA Saphire E.O.;
RT "Crystal Structure of the Marburg Virus VP35 Oligomerization Domain.";
RL J. Virol. 91:0-0(2017).
CC -!- FUNCTION: Plays an essential role in viral RNA synthesis and also a
CC role in suppressing innate immune signaling.
CC {ECO:0000269|PubMed:16282487, ECO:0000269|PubMed:23028316,
CC ECO:0000269|PubMed:30044983}.
CC -!- SUBUNIT: Homooligomer. Homomultimerization via the coiled coil domain
CC is a prerequisite for binding to L. Found in a trimeric complex in
CC which VP35 bridges L and the nucleoprotein (PubMed:16282487). Interacts
CC with NP (By similarity). Disrupts innate immune signaling in infected
CC host cell (PubMed:30044983, PubMed:23028316).
CC {ECO:0000250|UniProtKB:Q6UY68, ECO:0000269|PubMed:16282487,
CC ECO:0000269|PubMed:23028316, ECO:0000269|PubMed:30044983}.
CC -!- SUBCELLULAR LOCATION: Virion. Host cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the filoviridae polymerase cofactor VP35 family.
CC {ECO:0000255|PROSITE-ProRule:PRU01071, ECO:0000305}.
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DR EMBL; Z12132; CAA78115.1; -; mRNA.
DR EMBL; AY430365; AAR85461.1; -; Genomic_RNA.
DR EMBL; AY430366; AAR85454.1; -; Genomic_RNA.
DR EMBL; DQ217792; ABA87125.1; -; Genomic_RNA.
DR RefSeq; YP_001531154.1; NC_001608.3.
DR PDB; 4GH9; X-ray; 1.65 A; A=204-329.
DR PDB; 4GHA; X-ray; 2.50 A; A/C/E/G=204-329.
DR PDB; 5TOH; X-ray; 2.01 A; A/B/C=60-130.
DR PDB; 5TOI; X-ray; 2.19 A; A/B/C=60-130.
DR PDBsum; 4GH9; -.
DR PDBsum; 4GHA; -.
DR PDBsum; 5TOH; -.
DR PDBsum; 5TOI; -.
DR SMR; P35259; -.
DR DIP; DIP-60094N; -.
DR DNASU; 920948; -.
DR GeneID; 920948; -.
DR KEGG; vg:920948; -.
DR Proteomes; UP000007771; Genome.
DR Proteomes; UP000137266; Genome.
DR Proteomes; UP000160614; Genome.
DR Proteomes; UP000180448; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0019013; C:viral nucleocapsid; IDA:CACAO.
DR Gene3D; 1.10.8.950; -; 1.
DR Gene3D; 2.10.10.70; -; 1.
DR InterPro; IPR002953; Filo_VP35.
DR InterPro; IPR031163; VP35_IID.
DR InterPro; IPR043061; VP35_IID_b-sht.
DR InterPro; IPR043060; VP35_IID_hlx.
DR Pfam; PF02097; Filo_VP35; 1.
DR PIRSF; PIRSF018326; VP35_FiloV; 1.
DR PRINTS; PR01240; FILOVP35.
DR PROSITE; PS51735; VP35_IID; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Host cytoplasm; Reference proteome;
KW Transcription; Viral RNA replication; Virion.
FT CHAIN 1..329
FT /note="Polymerase cofactor VP35"
FT /id="PRO_0000222162"
FT DOMAIN 204..329
FT /note="VP35 IID"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01071"
FT COILED 70..120
FT /evidence="ECO:0000255"
FT VARIANT 31
FT /note="E -> K (in strain: pp3/guinea pig lethal and pp4/
FT guinea pig nonlethal)"
FT VARIANT 296
FT /note="S -> C (in strain: pp3/guinea pig lethal, pp4/guinea
FT pig nonlethal and Isolate Enterlein)"
FT MUTAGEN 90
FT /note="L->A: Complete loss of homo-oligomerization; when
FT associated with A-104."
FT /evidence="ECO:0000269|PubMed:16282487"
FT MUTAGEN 104
FT /note="L->A: Complete loss of homo-oligomerization; when
FT associated with A-90."
FT /evidence="ECO:0000269|PubMed:16282487"
FT HELIX 61..75
FT /evidence="ECO:0007829|PDB:5TOH"
FT HELIX 77..105
FT /evidence="ECO:0007829|PDB:5TOH"
FT HELIX 111..127
FT /evidence="ECO:0007829|PDB:5TOH"
FT HELIX 210..218
FT /evidence="ECO:0007829|PDB:4GH9"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 227..241
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 245..257
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 262..272
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 274..276
FT /evidence="ECO:0007829|PDB:4GH9"
FT STRAND 283..285
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 289..291
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 294..299
FT /evidence="ECO:0007829|PDB:4GH9"
FT HELIX 309..311
FT /evidence="ECO:0007829|PDB:4GH9"
FT STRAND 313..318
FT /evidence="ECO:0007829|PDB:4GH9"
FT STRAND 324..328
FT /evidence="ECO:0007829|PDB:4GH9"
SQ SEQUENCE 329 AA; 36143 MW; 300EDE165A40938E CRC64;
MWDSSYMQQV SEGLMTGKVP IDQVFGANPL EKLYKRRKPK GTVGLQCSPC LMSKATSTDD
IIWDQLIVKR TLADLLIPIN RQISDIQSTL SEVTTRVHEI ERQLHEITPV LKMGRTLEAI
SKGMSEMLAK YDHLVISTGR TTAPAAAFDA YLNEHGVPPP QPAIFKDLGV AQQACSKGTM
VKNATTDAAD KMSKVLELSE ETFSKPNLSA KDLALLLFTH LPGNNTPFHI LAQVLSKIAY
KSGKSGAFLD AFHQILSEGE NAQAALTRLS RTFDAFLGVV PPVIRVKNFQ TVPRPSQKSL
RAVPPNPTID KGWVCVYSSE QGETRALKI
