VP37A_MOUSE
ID VP37A_MOUSE Reviewed; 397 AA.
AC Q8CHS8; Q0VGD1; Q3TBI5; Q3TC15; Q3TC26;
DT 15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Vacuolar protein sorting-associated protein 37A;
DE Short=Vps37A;
DE AltName: Full=ESCRT-I complex subunit VPS37A;
GN Name=Vps37a;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Bone;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Liver, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the ESCRT-I complex, a regulator of vesicular
CC trafficking process. Required for the sorting of endocytic
CC ubiquitinated cargos into multivesicular bodies. May be involved in
CC cell growth and differentiation (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of the ESCRT-I complex (endosomal sorting complex
CC required for transport I) which consists of TSG101, VPS28, a VPS37
CC protein (VPS37A to -D) and MVB12A or MVB12B in a 1:1:1:1 stoichiometry.
CC Interacts with TSG101, VPS28 and HGS. Component of an ESCRT-I complex
CC (endosomal sorting complex required for transport I) which consists of
CC TSG101, VPS28, VPS37A and UBAP1 in a 1:1:1:1 stoichiometry (By
CC similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Late endosome membrane {ECO:0000250}; Peripheral
CC membrane protein {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8CHS8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CHS8-2; Sequence=VSP_025370, VSP_025371;
CC Name=3;
CC IsoId=Q8CHS8-3; Sequence=VSP_025372;
CC -!- SIMILARITY: Belongs to the VPS37 family. {ECO:0000305}.
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DR EMBL; AK170943; BAE42131.1; -; mRNA.
DR EMBL; AK170961; BAE42142.1; -; mRNA.
DR EMBL; AK171055; BAE42215.1; -; mRNA.
DR EMBL; AK171221; BAE42324.1; -; mRNA.
DR EMBL; BC039290; AAH39290.1; -; mRNA.
DR EMBL; BC110396; AAI10397.1; -; mRNA.
DR CCDS; CCDS22254.1; -. [Q8CHS8-1]
DR RefSeq; NP_291038.2; NM_033560.3. [Q8CHS8-1]
DR AlphaFoldDB; Q8CHS8; -.
DR SMR; Q8CHS8; -.
DR BioGRID; 206532; 2.
DR STRING; 10090.ENSMUSP00000096415; -.
DR iPTMnet; Q8CHS8; -.
DR PhosphoSitePlus; Q8CHS8; -.
DR EPD; Q8CHS8; -.
DR MaxQB; Q8CHS8; -.
DR PaxDb; Q8CHS8; -.
DR PeptideAtlas; Q8CHS8; -.
DR PRIDE; Q8CHS8; -.
DR ProteomicsDB; 297926; -. [Q8CHS8-1]
DR ProteomicsDB; 297927; -. [Q8CHS8-2]
DR ProteomicsDB; 297928; -. [Q8CHS8-3]
DR Antibodypedia; 22259; 153 antibodies from 24 providers.
DR DNASU; 52348; -.
DR Ensembl; ENSMUST00000098817; ENSMUSP00000096415; ENSMUSG00000031600. [Q8CHS8-1]
DR Ensembl; ENSMUST00000238339; ENSMUSP00000158798; ENSMUSG00000031600. [Q8CHS8-3]
DR GeneID; 52348; -.
DR KEGG; mmu:52348; -.
DR UCSC; uc009lmv.1; mouse. [Q8CHS8-1]
DR UCSC; uc009lmx.1; mouse. [Q8CHS8-3]
DR CTD; 137492; -.
DR MGI; MGI:1261835; Vps37a.
DR VEuPathDB; HostDB:ENSMUSG00000031600; -.
DR eggNOG; KOG3270; Eukaryota.
DR GeneTree; ENSGT00950000183012; -.
DR HOGENOM; CLU_062319_0_0_1; -.
DR InParanoid; Q8CHS8; -.
DR OMA; HIKECLR; -.
DR PhylomeDB; Q8CHS8; -.
DR TreeFam; TF332146; -.
DR Reactome; R-MMU-917729; Endosomal Sorting Complex Required For Transport (ESCRT).
DR BioGRID-ORCS; 52348; 17 hits in 73 CRISPR screens.
DR ChiTaRS; Vps37a; mouse.
DR PRO; PR:Q8CHS8; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q8CHS8; protein.
DR Bgee; ENSMUSG00000031600; Expressed in hindlimb stylopod muscle and 253 other tissues.
DR Genevisible; Q8CHS8; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0000813; C:ESCRT I complex; ISS:UniProtKB.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0031902; C:late endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR GO; GO:0043162; P:ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway; ISS:UniProtKB.
DR Gene3D; 1.10.287.660; -; 1.
DR Gene3D; 3.10.110.10; -; 1.
DR InterPro; IPR037202; ESCRT_assembly_dom.
DR InterPro; IPR029012; Helix_hairpin_bin_sf.
DR InterPro; IPR009851; Mod_r.
DR InterPro; IPR016135; UBQ-conjugating_enzyme/RWD.
DR Pfam; PF07200; Mod_r; 1.
DR SUPFAM; SSF140111; SSF140111; 1.
DR SUPFAM; SSF54495; SSF54495; 1.
DR PROSITE; PS51314; VPS37_C; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Endosome; Membrane; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..397
FT /note="Vacuolar protein sorting-associated protein 37A"
FT /id="PRO_0000287199"
FT DOMAIN 308..397
FT /note="VPS37 C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00646"
FT MOD_RES 18
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8NEZ2"
FT VAR_SEQ 324..346
FT /note="SCSASALQARLKVAAHEAEEESD -> VGLMSSVCPQELLSHRSCPALCI
FT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025370"
FT VAR_SEQ 347..397
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_025371"
FT VAR_SEQ 372..397
FT /note="ICHCRRAKEEKLHQVIAMHSQFHAPL -> VCNTLQLRISDNIITIFLSMFI
FT SKHR (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_025372"
FT CONFLICT 14
FT /note="S -> P (in Ref. 1; BAE42131)"
FT /evidence="ECO:0000305"
FT CONFLICT 119
FT /note="L -> P (in Ref. 1; BAE42324)"
FT /evidence="ECO:0000305"
FT CONFLICT 193
FT /note="A -> E (in Ref. 1; BAE42324)"
FT /evidence="ECO:0000305"
FT CONFLICT 203
FT /note="P -> L (in Ref. 1; BAE42324)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 397 AA; 44419 MW; 128162E458A8746A CRC64;
MSWLFPLAKS ASSSAAGSPA GLTSLQQQKQ RLIESLRNSH SSIAEIQKDV EYRLPFTVNN
LTININILLP PQFPQEKPVI SVYPPIRHHL MDSQGLYVTS PLVSNFTMHS DLGKIIQSLL
DEFWKNPPVL APTSTTFPYL YSNPGGMPPY PSQGFPFLPP YPPPEANRNI TSLSVADTVS
SSTTSYTAAK PVAPSFGILS SLPLPVPTTE SSASVNQNGF GYKMPDIPDA FPELSELSVS
QLTDMNEQEE VLLEQFLMLP QLKQIITDKE DLVKNIEELA RKNLLLEHSL EGKRQTVLDK
YELLLQMKST FEKKMQRQHE LSESCSASAL QARLKVAAHE AEEESDNIAE DFLEGKTEID
DFLNSFKEKR TICHCRRAKE EKLHQVIAMH SQFHAPL
