ID   VP3_CAVC1               Reviewed;         121 AA.
AC   Q99152;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   02-JUN-2021, entry version 78.
DE   RecName: Full=Apoptin;
GN   Name=VP3;
OS   Chicken anemia virus (isolate Germany Cuxhaven-1) (CAV).
OC   Viruses; Anelloviridae; Gyrovirus.
OX   NCBI_TaxID=73475;
OH   NCBI_TaxID=9031; Gallus gallus (Chicken).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1851873; DOI=10.1128/jvi.65.6.3131-3139.1991;
RA   Noteborn M.H.M., de Boer G.F., van Roozelaar D.J., Karreman C.,
RA   Kranenburg O., Vos J.G., Jeurissen S.H.M., Hoeben R.C., Zantema A.,
RA   Koch G., van Ormondt H., van der Eb A.J.;
RT   "Characterization of cloned chicken anemia virus DNA that contains all
RT   elements for the infectious replication cycle.";
RL   J. Virol. 65:3131-3139(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1605740; DOI=10.1007/bf01309811;
RA   Meehan B.M., Todd D., Creelan J.L., Earle J.A.P., Hoey E.M., McNulty M.S.;
RT   "Characterization of viral DNAs from cells infected with chicken anaemia
RT   agent: sequence analysis of the cloned replicative form and transfection
RT   capabilities of cloned genome fragments.";
RL   Arch. Virol. 124:301-319(1992).
RN   [3]
RP   INDUCTION.
RX   PubMed=9049362; DOI=10.1099/0022-1317-76-7-1557;
RA   Douglas A.J., Phenix K., Mawhinney K.A., Todd D., Mackie D.P., Curran W.L.;
RT   "Identification of a 24 kDa protein expressed by chicken anaemia virus.";
RL   J. Gen. Virol. 76:1557-1562(1995).
CC   -!- FUNCTION: May act as transcriptional regulator. Induces apoptosis in
CC       infected cells. Element of infectious replication cycle.
CC   -!- INTERACTION:
CC       Q99152; P27986: PIK3R1; Xeno; NbExp=3; IntAct=EBI-1776808, EBI-79464;
CC       Q99152; Q9H2H8: PPIL3; Xeno; NbExp=3; IntAct=EBI-1776808, EBI-751051;
CC   -!- SUBCELLULAR LOCATION: Host nucleus. Note=Host nucleus of infected
CC       cells.
CC   -!- INDUCTION: VP1 and VP2 are detected 12 hours post infection, while VP3
CC       only after 24 hours. {ECO:0000269|PubMed:9049362}.
CC   -!- SIMILARITY: Belongs to the gyrovirus apoptin family. {ECO:0000305}.
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DR   EMBL; M55918; AAA91823.1; -; Genomic_DNA.
DR   EMBL; M81223; AAA42883.1; -; Genomic_DNA.
DR   PIR; B39926; B39926.
DR   PIR; B48343; B48343.
DR   RefSeq; NP_056774.1; NC_001427.1.
DR   DIP; DIP-45593N; -.
DR   IntAct; Q99152; 4.
DR   MINT; Q99152; -.
DR   GeneID; 1494446; -.
DR   Proteomes; UP000007528; Genome.
DR   GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0019051; P:induction by virus of host apoptotic process; IEA:InterPro.
DR   GO; GO:0039593; P:suppression by virus of host exit from mitosis; IEA:UniProtKB-KW.
DR   InterPro; IPR006858; CAV_VP3.
DR   Pfam; PF04771; CAV_VP3; 1.
PE   1: Evidence at protein level;
KW   Apoptosis; Early protein; Host nucleus; Host-virus interaction;
KW   Inhibition of host mitotic exit by virus;
KW   Modulation of host cell cycle by virus; Reference proteome.
FT   CHAIN           1..121
FT                   /note="Apoptin"
FT                   /id="PRO_0000223008"
FT   REGION          1..28
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          57..121
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        57..74
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        80..98
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        70
FT                   /note="F -> S (in Ref. 2; AAA42883)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        116
FT                   /note="K -> R (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        118
FT                   /note="R -> C (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   121 AA;  13270 MW;  D2AAB3869BC12A3E CRC64;
     MNALQEDTPP GPSTVFRPPT SSRPLETPHC REIRIGIAGI TITLSLCGCA NARAPTLRSA
     TADNSESTGF KNVPDLRTDQ PKPPSKKRSC DPSEYRVSEL KESLITTTPS RPRTAKRRIR
     L