ID   VP53B_ARATH             Reviewed;         569 AA.
AC   F4I7Y2; Q683B5; Q9C6I3;
DT   22-JAN-2014, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Vacuolar protein sorting-associated protein 53 B;
GN   OrderedLocusNames=At1g50970; ORFNames=F8A12.19;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in retrograde transport from early and late
CC       endosomes to late Golgi, leading to the membrane fusion between late
CC       Golgi and endosomal vesicles. {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Golgi-associated retrograde protein (GARP)
CC       complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus, trans-Golgi network
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}.
CC       Endosome membrane {ECO:0000250}; Peripheral membrane protein
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=F4I7Y2-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=F4I7Y2-2; Sequence=VSP_053515, VSP_053516;
CC   -!- SIMILARITY: Belongs to the VPS53 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG50948.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC079284; AAG50948.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE32607.1; -; Genomic_DNA.
DR   EMBL; AK175202; BAD42965.1; -; mRNA.
DR   PIR; H96546; H96546.
DR   RefSeq; NP_175510.2; NM_103977.2. [F4I7Y2-1]
DR   AlphaFoldDB; F4I7Y2; -.
DR   SMR; F4I7Y2; -.
DR   BioGRID; 26744; 2.
DR   STRING; 3702.AT1G50970.1; -.
DR   PaxDb; F4I7Y2; -.
DR   PRIDE; F4I7Y2; -.
DR   EnsemblPlants; AT1G50970.1; AT1G50970.1; AT1G50970. [F4I7Y2-1]
DR   GeneID; 841519; -.
DR   Gramene; AT1G50970.1; AT1G50970.1; AT1G50970. [F4I7Y2-1]
DR   KEGG; ath:AT1G50970; -.
DR   Araport; AT1G50970; -.
DR   TAIR; locus:2036396; AT1G50970.
DR   eggNOG; KOG2180; Eukaryota.
DR   HOGENOM; CLU_479281_0_0_1; -.
DR   InParanoid; F4I7Y2; -.
DR   OMA; AIHRESH; -.
DR   OrthoDB; 1379600at2759; -.
DR   PRO; PR:F4I7Y2; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; F4I7Y2; baseline and differential.
DR   Genevisible; F4I7Y2; AT.
DR   GO; GO:0005829; C:cytosol; IEA:GOC.
DR   GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0000938; C:GARP complex; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0042147; P:retrograde transport, endosome to Golgi; IBA:GO_Central.
DR   InterPro; IPR039766; Vps53.
DR   InterPro; IPR007234; Vps53_N.
DR   PANTHER; PTHR12820; PTHR12820; 3.
DR   Pfam; PF04100; Vps53_N; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Coiled coil; Cytoplasm; Endosome; Golgi apparatus;
KW   Membrane; Protein transport; Reference proteome; Transport.
FT   CHAIN           1..569
FT                   /note="Vacuolar protein sorting-associated protein 53 B"
FT                   /id="PRO_0000424846"
FT   COILED          53..90
FT                   /evidence="ECO:0000255"
FT   COILED          125..145
FT                   /evidence="ECO:0000255"
FT   COILED          295..316
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         142..158
FT                   /note="AINELCNHFKAYMDLPK -> LRYWDRDRGIVFAKEVI (in isoform
FT                   2)"
FT                   /evidence="ECO:0000303|Ref.3"
FT                   /id="VSP_053515"
FT   VAR_SEQ         159..569
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.3"
FT                   /id="VSP_053516"
SQ   SEQUENCE   569 AA;  64125 MW;  317E6BDDA05B5AFD CRC64;
     MDKSSGLDFI NQMFPVEASL SCVESFMQKI CDEIRRVDAT ILAVVSQQGN SGTRAKENLN
     DAICAAEELS HKIQEIKSKA EQTEAMVQDI CSDIKKLDFA KKNITTAVTA LSRLTMLVSA
     VQQLQVMTSK RQYKEAATQL EAINELCNHF KAYMDLPKIM ELREKLKNIK QILKFHVFSD
     FSSLGTGTET EELFLLKKLS DSCLVVDALE PSVREELINN FCSRELTSYE QIYVGAELKT
     LDEIELIYNQ LSCLIRKNQG KWTIFPASWH VPYRLCIQLS RKTRVQVESI LVNLKEKSDV
     EKLLLELKRT LEFERELEMK FGGGGSIGDD IIGGGGNNSQ KFNFRGMISS CFEPHLTIYI
     EKEEMELMQL LEKVVQEETW DIEEELGCHS ENSVYLMLLD AHDMKMILLK VPSLARQPEA
     SALLVKTATA SYVKLVNHQM KRAEAVLKVI ASPIVTVIDT YRALFPEETP MEFQRILVLK
     GLTKAEQQSI LDDFNNHSSR ITQLSVAAKT PEAHALPLAL TNVAPAVRFK ANSEEVLTRA
     ASAATTSFMK LYALTGAAKD RPFRKLFNP