ID   VP5_AHSV4               Reviewed;         505 AA.
AC   Q02168; Q64926;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=Outer capsid protein VP5;
GN   Name=Segment-6; Synonyms=M6;
OS   African horse sickness virus 4 (AHSV-4).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Orbivirus.
OX   NCBI_TaxID=36421;
OH   NCBI_TaxID=9838; Camelus dromedarius (Dromedary) (Arabian camel).
OH   NCBI_TaxID=9615; Canis lupus familiaris (Dog) (Canis familiaris).
OH   NCBI_TaxID=9793; Equus asinus (Donkey) (Equus africanus asinus).
OH   NCBI_TaxID=9796; Equus caballus (Horse).
OH   NCBI_TaxID=9794; Equus hemionus (Onager) (Asian wild ass).
OH   NCBI_TaxID=89252; Equus quagga burchellii (Plains zebra) (Equus burchelli).
OH   NCBI_TaxID=9785; Loxodonta africana (African elephant).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Vaccine;
RX   PubMed=1329319; DOI=10.1016/0042-6822(92)90187-t;
RA   Iwata H., Yamagawa M., Roy P.;
RT   "Evolutionary relationships among the gnat-transmitted orbiviruses that
RT   cause African horse sickness, bluetongue, and epizootic hemorrhagic disease
RT   as evidenced by their capsid protein sequences.";
RL   Virology 191:251-261(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Vaccine;
RA   Iwata H., Yamagawa M., Roy P.;
RL   Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Vaccine;
RX   PubMed=8075221; DOI=10.1292/jvms.56.321;
RA   Sakamoto K., Mizukoshi N., Apiwatnakorn B., Iwata A., Tsuchiya T., Ueda S.,
RA   Imagawa H., Sugiura T., Kamada M., Fukusho A.;
RT   "The complete sequences of African horsesickness virus serotype 4 (vaccine
RT   strain) RNA segment 2 and 6 which encode outer capsid protein.";
RL   J. Vet. Med. Sci. 56:321-327(1994).
CC   -!- FUNCTION: VP5 protein is one of the two proteins (with VP2) which
CC       constitute the virus particle outer capsid. Acts as a membrane
CC       permeabilization protein that mediates release of viral particles from
CC       endosomal compartments into the cytoplasm. Permeabilization activity is
CC       probably negatively regulated by VP2 and is triggered by endosomal
CC       degradation of VP2 and exposure to low pH (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orbivirus VP5 family. {ECO:0000305}.
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DR   EMBL; M94682; AAA42539.1; -; Genomic_RNA.
DR   EMBL; M94731; AAA92778.1; -; Genomic_RNA.
DR   EMBL; D26571; BAA05620.1; -; Genomic_RNA.
DR   PIR; A44053; P5XRA4.
DR   RefSeq; YP_052963.1; NC_006018.1.
DR   SMR; Q02168; -.
DR   GeneID; 2930878; -.
DR   KEGG; vg:2930878; -.
DR   GO; GO:0039624; C:viral outer capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0039665; P:permeabilization of host organelle membrane involved in viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0099008; P:viral entry via permeabilization of inner membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR000145; Capsid_VP5_Orbivir.
DR   Pfam; PF00901; Orbi_VP5; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Outer capsid protein;
KW   Viral penetration into host cytoplasm;
KW   Viral penetration via permeabilization of host membrane; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..505
FT                   /note="Outer capsid protein VP5"
FT                   /id="PRO_0000222716"
FT   REGION          1..42
FT                   /note="Involved in membrane permeabilization"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        13
FT                   /note="N -> S (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        17
FT                   /note="R -> K (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        28
FT                   /note="Y -> H (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        46
FT                   /note="A -> P (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        444
FT                   /note="R -> K (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        501
FT                   /note="G -> R (in Ref. 2; BAA05620)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   505 AA;  56780 MW;  DA2930C3E899D317 CRC64;
     MGKFTSFLKR AGNATKRALT SDSAKKMYKL AGKTLQRVVE SEVGSAAIDG VMQGAIQSII
     QGENLGDSIK QAVILNVAGT LESAPDPLSP GEQLLYNKVS EIEKMEKEDR VIETHNAKIE
     EKFGKDLLAI RKIVKGEVDA EKLEGNEIKY VEKALSGLLE IGKDQSERIT KLYRALQTEE
     DLRTRDETRM INEYREKFDA LKEAIEIEQQ ATHDEAIQEM LDLSAEVIET ASEEVPIFGA
     GAANVIATTR AIQGGLKLKE IVDKLTGIDL SHLKVADIHP HIIEKAMLRD TVTDKDLAMA
     IKSKVDVIDE MNVETQHVID AVLPIVKQEY EKHDNKYHVR IPGALKIHSE HTPKIHIYTT
     PWDSDSVFMC RAIAPHHQQR SFFIGFDLEI EYVHFEDTSV EGHILHGGAI TVEGRGFRQA
     YTEFMNAAWG MPTTPELHKR KLQRSMGTHP IYMGSMDYAI SYEQLVSNAM RLVYDSELQM
     HCLRGPLKFQ RRTLMNALLY GVKIA