ID   VP5_BTV10               Reviewed;         526 AA.
AC   P07389;
DT   01-APR-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1988, sequence version 1.
DT   02-DEC-2020, entry version 70.
DE   RecName: Full=Outer capsid protein VP5;
GN   Name=Segment-6; Synonyms=M5;
OS   Bluetongue virus 10 (isolate USA) (BTV 10).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Orbivirus.
OX   NCBI_TaxID=10900;
OH   NCBI_TaxID=9891; Antilocapra americana (Pronghorn).
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=46212; Culicoides variipennis (Biting midge).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3009695; DOI=10.1099/0022-1317-67-5-957;
RA   Purdy M.A., Ritter G.D., Roy P.;
RT   "Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5,
RT   of bluetongue virus serotype 10.";
RL   J. Gen. Virol. 67:957-962(1986).
RN   [2]
RP   FUNCTION.
RX   PubMed=14762165; DOI=10.1073/pnas.0306448101;
RA   Forzan M., Wirblich C., Roy P.;
RT   "A capsid protein of nonenveloped Bluetongue virus exhibits membrane
RT   Permeabilization activity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:2100-2105(2004).
CC   -!- FUNCTION: VP5 protein is one of the two proteins (with VP2) which
CC       constitute the virus particle outer capsid. Acts as a membrane
CC       permeabilization protein that mediates release of viral particles from
CC       endosomal compartments into the cytoplasm. Permeabilization activity is
CC       probably negatively regulated by VP2 and is triggered by endosomal
CC       degradation of VP2 and exposure to low pH.
CC       {ECO:0000269|PubMed:14762165}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the orbivirus VP5 family. {ECO:0000305}.
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DR   EMBL; D12532; BAA02095.1; -; Genomic_RNA.
DR   PIR; A25419; P5XR10.
DR   RefSeq; YP_052955.1; NC_006010.1.
DR   SMR; P07389; -.
DR   GeneID; 2943151; -.
DR   KEGG; vg:2943151; -.
DR   Proteomes; UP000007662; Genome.
DR   GO; GO:0039624; C:viral outer capsid; IEA:UniProtKB-KW.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   GO; GO:0039665; P:permeabilization of host organelle membrane involved in viral entry into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0099008; P:viral entry via permeabilization of inner membrane; IEA:UniProtKB-KW.
DR   InterPro; IPR000145; Capsid_VP5_Orbivir.
DR   Pfam; PF00901; Orbi_VP5; 1.
PE   3: Inferred from homology;
KW   Capsid protein; Outer capsid protein; Reference proteome;
KW   Viral penetration into host cytoplasm;
KW   Viral penetration via permeabilization of host membrane; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..526
FT                   /note="Outer capsid protein VP5"
FT                   /id="PRO_0000222712"
FT   REGION          1..42
FT                   /note="Involved in membrane permeabilization"
SQ   SEQUENCE   526 AA;  59163 MW;  29AE556DBD55522C CRC64;
     MGKIIKSLSR FGKKVGNALT SNTAKKIYST IGKAAERFAE SEIGAATIDG LVQGSVHSII
     TGESYGESVK QAVLLNVLGT GEELPDPLSP GERGMQTKIK ELEDEQRNEL VRLKYNKEIT
     EKFGKELGEV YDFMNGEAKE VEAVEEQYTM LCKAVDSYEK ILKEEDSKMA ILARALQREA
     AERSEDEIKM VKEYRQKIDA LKSAIEIERD GMQEEAIQEI AGMTADVLEA ASEEVPLIGA
     GMATAVATGR AIEGAYKLKK VINALSGIDL SHMRSPKIEP TIIATTLEHR FKDIPDEQLA
     ISVLNKKTAV ADNCNEIAHI KQEILPKFKQ IMNEEKEIEG IEDKVIHPRV MMRFKIPRTQ
     QPQIHIYAAP WDSDDVFFFH CVSYHHRNES FFLGFDLGID VVHFEDLTSH WHALGMAQEA
     SGRTLTEAYR EFLNLSISST FSSAIHARRM IRSRAVHPIF LGSMHYDITY EALKNNAQRI
     VYDDELQMHI LRGPLHFQRR AILGALKFGV KILGDKIDVP LFLRNA