ID   VP74L_DICDI             Reviewed;         262 AA.
AC   Q54J20;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=VPS74-like protein DDB_G0288371;
GN   ORFNames=DDB_G0288371;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Phosphatidylinositol-4-phosphate-binding protein that links
CC       Golgi membranes to the cytoskeleton and may participate in the tensile
CC       force required for vesicle budding from the Golgi. Thereby, may play a
CC       role in Golgi membrane trafficking. May also bind to the coatomer to
CC       regulate Golgi membrane trafficking. May play a role in anterograde
CC       transport from the Golgi to the plasma membrane and regulate secretion.
CC       May be involved in vacuolar protein sorting (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, Golgi stack membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the GOLPH3/VPS74 family. {ECO:0000305}.
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DR   EMBL; AAFI02000111; EAL63282.1; -; Genomic_DNA.
DR   RefSeq; XP_636781.1; XM_631689.1.
DR   AlphaFoldDB; Q54J20; -.
DR   SMR; Q54J20; -.
DR   STRING; 44689.DDB0234232; -.
DR   PaxDb; Q54J20; -.
DR   EnsemblProtists; EAL63282; EAL63282; DDB_G0288371.
DR   GeneID; 8626584; -.
DR   KEGG; ddi:DDB_G0288371; -.
DR   dictyBase; DDB_G0288371; -.
DR   eggNOG; KOG3983; Eukaryota.
DR   HOGENOM; CLU_1063293_0_0_1; -.
DR   InParanoid; Q54J20; -.
DR   OMA; NTTRYSM; -.
DR   PRO; PR:Q54J20; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0032580; C:Golgi cisterna membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0070273; F:phosphatidylinositol-4-phosphate binding; IEA:InterPro.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.3630.10; -; 1.
DR   InterPro; IPR008628; GPP34-like.
DR   InterPro; IPR038261; GPP34-like_sf.
DR   Pfam; PF05719; GPP34; 1.
PE   3: Inferred from homology;
KW   Golgi apparatus; Lipid-binding; Membrane; Protein transport;
KW   Reference proteome; Transport.
FT   CHAIN           1..262
FT                   /note="VPS74-like protein DDB_G0288371"
FT                   /id="PRO_0000393699"
SQ   SEQUENCE   262 AA;  29609 MW;  AE5FAC68AD7286DD CRC64;
     MNPDNDNDSL PLLTLPEQLF LLILNPETGK LPYTFVPLFH GFVGCGIAEL QLMDKVIVTK
     QNSQSFNNNK MLIKVIDHSK TGDTFLDYIL SKLSNSFAQK GLQMATAILT ISVGLNKMKR
     ITETIAQSLI KKGFIRGTLK RSVLFKNKSI YEVIDYQAKL SVESSICKVL STPPDYPDNC
     LRELIILLTF KQYEDFLIKP KLMDSLISRL YHPEQCAHIK ANLRLINKNF YKEPSEVHLT
     NDPSVKMLSL VIGGIRNAIT SE