ID   VP8_BTV2A               Reviewed;         229 AA.
AC   Q04687;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   12-AUG-2020, entry version 43.
DE   RecName: Full=Non-structural protein P8;
DE   AltName: Full=Non-structural protein NS3;
DE   Contains:
DE     RecName: Full=Non-structural protein NS3A;
GN   Name=Segment-10;
OS   Bluetongue virus 2 (isolate USA) (BTV 2).
OC   Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes;
OC   Reovirales; Reoviridae; Sedoreovirinae; Orbivirus.
OX   NCBI_TaxID=10907;
OH   NCBI_TaxID=9891; Antilocapra americana (Pronghorn).
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=9925; Capra hircus (Goat).
OH   NCBI_TaxID=46212; Culicoides variipennis (Biting midge).
OH   NCBI_TaxID=9940; Ovis aries (Sheep).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=1318624; DOI=10.1016/0168-1702(92)90074-j;
RA   Hwang G.-Y., Yang Y.-Y., Chiou J.-F., Li J.K.-K.;
RT   "Sequence conservation among the cognate nonstructural NS3/3A protein genes
RT   of six bluetongue viruses.";
RL   Virus Res. 23:151-161(1992).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immune
CC       response. Interacts with host OPTN and thus inhibits the recruitment of
CC       TBK1 to the host Golgi apparatus. In turn, downstream partner IRF3
CC       cannot be activated and IFN-beta production is impaired.
CC       {ECO:0000250|UniProtKB:P08363}.
CC   -!- FUNCTION: Facilitates viral particle release either by increasing
CC       plasma membrane permeability through a viroporin-like activity or by
CC       viral budding. {ECO:0000250|UniProtKB:P08363}.
CC   -!- SUBUNIT: Forms homooligomers via coiled-coil motif. Interacts with host
CC       OPTN; this interaction inhibits innate immune response.
CC       {ECO:0000250|UniProtKB:P08363}.
CC   -!- SUBCELLULAR LOCATION: Host cell membrane
CC       {ECO:0000250|UniProtKB:P08363}; Multi-pass membrane protein
CC       {ECO:0000255}. Host Golgi apparatus {ECO:0000250|UniProtKB:P08363}.
CC   -!- SIMILARITY: Belongs to the orbivirus NS3 family. {ECO:0000305}.
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DR   EMBL; L08628; AAA42834.1; -; Genomic_RNA.
DR   GO; GO:0044177; C:host cell Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0020002; C:host cell plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR002565; Orbi_NS3.
DR   Pfam; PF01616; Orbi_NS3; 1.
PE   3: Inferred from homology;
KW   Host cell membrane; Host Golgi apparatus; Host membrane;
KW   Host-virus interaction; Inhibition of host innate immune response by virus;
KW   Membrane; Transmembrane; Transmembrane helix; Viral immunoevasion.
FT   CHAIN           1..229
FT                   /note="Non-structural protein P8"
FT                   /id="PRO_0000040626"
FT   CHAIN           14..229
FT                   /note="Non-structural protein NS3A"
FT                   /id="PRO_0000040627"
FT   TRANSMEM        119..139
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        162..182
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   229 AA;  25482 MW;  74A93F8C21220C56 CRC64;
     MLSGLIQRFE EEKMKHNQER VEELSLVRVD DAISQPPRYA PSAPMPSSMP TVALEILDKA
     MSNTTGATQT QKAEKAAFAS YAEAFRDDVR LRQIKRHVNE QILPKLKSDL GGLKKKRAII
     HMTLLIAAVV ALLTSVCTLS SDMSVAFKLN GTSAEIPQWF KSLNPMLGVV NVGATFLMMV
     CAKSERSLNQ QIDMIKKEVM KKQSYNDAVR MSFTEFSSVP LDGFELPLT