ID   VP8_VACCC               Reviewed;         251 AA.
AC   P20981;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1991, sequence version 1.
DT   29-SEP-2021, entry version 63.
DE   RecName: Full=Core protein VP8;
DE   AltName: Full=25 kDa major core protein;
DE   AltName: Full=L4 core protein;
DE   AltName: Full=P25K;
DE   Flags: Precursor;
GN   ORFNames=L4R;
OS   Vaccinia virus (strain Copenhagen) (VACV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX   NCBI_TaxID=10249;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=2219722; DOI=10.1016/0042-6822(90)90294-2;
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "The complete DNA sequence of vaccinia virus.";
RL   Virology 179:247-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P.,
RA   Paoletti E.;
RT   "Appendix to 'The complete DNA sequence of vaccinia virus'.";
RL   Virology 179:517-563(1990).
CC   -!- FUNCTION: Major core structural protein. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Localizes to the
CC       virion core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: Undergoes morphogenesis-associated proteolysis which cleaves the
CC       28 kDa to a 25-kDa product. Proteolytic cleavage of major core proteins
CC       P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC       core formation, is required for production of infectious mature virions
CC       (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae VP8 family. {ECO:0000305}.
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DR   EMBL; M35027; AAA48079.1; -; Genomic_DNA.
DR   PIR; B42513; B42513.
DR   Proteomes; UP000008269; Genome.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR007586; VP8_pox_nuc-bd.
DR   Pfam; PF04498; Pox_VP8_L4R; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Reference proteome; Virion.
FT   PROPEP          1..32
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040589"
FT   CHAIN           33..251
FT                   /note="Core protein VP8"
FT                   /id="PRO_0000040590"
FT   SITE            18..19
FT                   /note="Cleavage; by core protease I7"
FT                   /evidence="ECO:0000250"
FT   SITE            32..33
FT                   /note="Cleavage; by core protease I7"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   251 AA;  28461 MW;  BA34200CFBC045B1 CRC64;
     MSLLLENLIE EDTIFFAGSI SEYDDLQMVI AGAKSKFPRS MLSIFNIVPR TMSKYELELI
     HNENITGAMF TTMYNIRNNL GLGDDKLTIE AIENYFLDPN NEVMPLIINN TDMTAVIPKK
     SGRRKNKNMV IFRQGSSPIL CIFETRKKIN IYKENMESAS TEYTPIGDNK ALISKYAGIN
     VLNVYSPSTS MRLNAIYGFT NKNKLEKLST NKELESYSSS PLQEPIRLND FLGLLECVKK
     NIPLTDIPTK D