VP8_VACCW
ID VP8_VACCW Reviewed; 251 AA.
AC P03295; Q76ZT5;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1988, sequence version 1.
DT 23-FEB-2022, entry version 77.
DE RecName: Full=Core protein VP8;
DE AltName: Full=25 kDa major core protein;
DE AltName: Full=F5 polypeptide;
DE AltName: Full=L4 core protein;
DE AltName: Full=P25K;
DE Flags: Precursor;
GN OrderedLocusNames=VACWR091; ORFNames=L4R;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2987815; DOI=10.1093/nar/13.3.985;
RA Plucienniczak A., Schroeder E., Zettlmeissl G., Streeck R.E.;
RT "Nucleotide sequence of a cluster of early and late genes in a conserved
RT segment of the vaccinia virus genome.";
RL Nucleic Acids Res. 13:985-998(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6088791; DOI=10.1128/jvi.51.3.662-669.1984;
RA Weir J.P., Moss B.;
RT "Regulation of expression and nucleotide sequence of a late vaccinia virus
RT gene.";
RL J. Virol. 51:662-669(1984).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3903212; DOI=10.1128/jvi.56.2.534-540.1985;
RA Weir J.P., Moss B.;
RT "Use of a bacterial expression vector to identify the gene encoding a major
RT core protein of vaccinia virus.";
RL J. Virol. 56:534-540(1985).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 33-52, PROTEOLYTIC CLEAVAGE, AND INDUCTION.
RX PubMed=3201753;
RA Yang W.-P., Kao S.-Y., Bauer W.R.;
RT "Biosynthesis and post-translational cleavage of vaccinia virus structural
RT protein VP8.";
RL Virology 167:585-590(1988).
RN [6]
RP PROTEIN SEQUENCE OF 33-41.
RX PubMed=1993877; DOI=10.1099/0022-1317-72-2-411;
RA van Slyke J.K., Franke C.A., Hruby D.E.;
RT "Proteolytic maturation of vaccinia virus core proteins: identification of
RT a conserved motif at the N termini of the 4b and 25K virion proteins.";
RL J. Gen. Virol. 72:411-416(1991).
RN [7]
RP SUBCELLULAR LOCATION, AND DNA-BINDING.
RX PubMed=3201752;
RA Yang W.P., Bauer W.R.;
RT "Purification and characterization of vaccinia virus structural protein
RT VP8.";
RL Virology 167:578-584(1988).
RN [8]
RP RNA-BINDING, AND DNA-BINDING.
RX PubMed=9321647; DOI=10.1093/nar/25.20.3984;
RA Bayliss C.D., Smith G.L.;
RT "Vaccinia virion protein VP8, the 25 kDa product of the L4R gene, binds
RT single-stranded DNA and RNA with similar affinity.";
RL Nucleic Acids Res. 25:3984-3990(1997).
RN [9]
RP PROTEOLYTIC CLEAVAGE, AND MUTAGENESIS OF GLY-18 AND GLY-32.
RX PubMed=15163727; DOI=10.1128/jvi.78.12.6335-6343.2004;
RA Ansarah-Sobrinho C., Moss B.;
RT "Role of the I7 protein in proteolytic processing of vaccinia virus
RT membrane and core components.";
RL J. Virol. 78:6335-6343(2004).
CC -!- FUNCTION: Major core structural protein.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:3201752}.
CC Note=Localizes to the virion core.
CC -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:3201753}.
CC -!- PTM: Undergoes morphogenesis-associated proteolysis which cleaves the
CC 28 kDa to a 25-kDa product. Proteolytic cleavage of major core proteins
CC P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC core formation, is required for production of infectious mature virions
CC (MV). {ECO:0000269|PubMed:15163727, ECO:0000269|PubMed:3201753}.
CC -!- SIMILARITY: Belongs to the chordopoxvirinae VP8 family. {ECO:0000305}.
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DR EMBL; X01978; CAA26013.1; -; Genomic_DNA.
DR EMBL; M11758; AAA48232.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89370.1; -; Genomic_DNA.
DR PIR; A03867; WMVZ28.
DR PIR; D23092; WMVZF5.
DR RefSeq; YP_232973.1; NC_006998.1.
DR DIP; DIP-2168N; -.
DR IntAct; P03295; 1.
DR MINT; P03295; -.
DR DNASU; 3707547; -.
DR GeneID; 3707547; -.
DR KEGG; vg:3707547; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR InterPro; IPR007586; VP8_pox_nuc-bd.
DR Pfam; PF04498; Pox_VP8_L4R; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Late protein; Reference proteome; Virion.
FT PROPEP 1..32
FT /note="Removed by core protease I7"
FT /evidence="ECO:0000269|PubMed:1993877,
FT ECO:0000269|PubMed:3201753"
FT /id="PRO_0000040591"
FT CHAIN 33..251
FT /note="Core protein VP8"
FT /id="PRO_0000040592"
FT SITE 18..19
FT /note="Cleavage; by core protease I7"
FT /evidence="ECO:0000305"
FT SITE 32..33
FT /note="Cleavage; by core protease I7"
FT MUTAGEN 18
FT /note="G->A: No effect on cleavage by core protease I7."
FT /evidence="ECO:0000269|PubMed:15163727"
FT MUTAGEN 32
FT /note="G->A: Complete loss of cleavage by core protease
FT I7."
FT /evidence="ECO:0000269|PubMed:15163727"
FT CONFLICT 144
FT /note="E -> Q (in Ref. 3; AAA48232)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 251 AA; 28457 MW; 7FEC9BAFFFC45737 CRC64;
MSLLLENLIE EDTIFFAGSI SEYDDLQMVI AGAKSKFPRS MLSIFNIVPR TMSKYELELI
HNENITGAMF TTMYNIRNNL GLGDDKLTIE AIENYFLDPN NEVMPLIINN TDMTAVIPKK
SGRRKNKNMV IFRQGSSPIL CIFETRKKIN IYKENMESAS TEYTPIGDNK ALISKYAGIN
ILNVYSPSTS IRLNAIYGFT NKNKLEKLST NKELESYSSS PLQEPIRLND FLGLLECVKK
NIPLTDIPTK D
