ID   VP8_VAR67               Reviewed;         251 AA.
AC   P0DSW9; P33039;
DT   16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-OCT-2019, sequence version 1.
DT   12-AUG-2020, entry version 6.
DE   RecName: Full=Core protein VP8;
DE   AltName: Full=25 kDa major core protein;
DE   AltName: Full=L4 core protein;
DE   AltName: Full=P25K;
DE   Flags: Precursor;
GN   ORFNames=L4R;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8383392; DOI=10.1016/0168-1702(93)90110-9;
RA   Shchelkunov S.N., Blinov V.M., Totmenin A.V., Marennikova S.S.,
RA   Kolykhalov A.A., Frolov I.V., Chizhikov V.E., Gytorov V.V., Gashikov P.V.,
RA   Belanov E.F., Belavin P.A., Resenchuk S.M., Andzhaparidze O.G.,
RA   Sandakhchiev L.S.;
RT   "Nucleotide sequence analysis of variola virus HindIII M, L, I genome
RT   fragments.";
RL   Virus Res. 27:25-35(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Major core structural protein. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250}. Note=Localizes to the
CC       virion core. {ECO:0000250}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC   -!- PTM: Undergoes morphogenesis-associated proteolysis which cleaves the
CC       28 kDa to a 25-kDa product. Proteolytic cleavage of major core proteins
CC       P4a (A10L), P4b (A3L), and VP8 (L4R), which occurs at a late stage of
CC       core formation, is required for production of infectious mature virions
CC       (MV) (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the chordopoxvirinae VP8 family. {ECO:0000305}.
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DR   EMBL; X67119; CAA47575.1; -; Genomic_DNA.
DR   EMBL; S55844; AAB24672.1; -; Genomic_DNA.
DR   EMBL; X69198; CAA49017.1; -; Genomic_DNA.
DR   PIR; A36845; A36845.
DR   RefSeq; NP_042120.1; NC_001611.1.
DR   GeneID; 1486473; -.
DR   KEGG; vg:1486473; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0019028; C:viral capsid; IEA:InterPro.
DR   GO; GO:0005198; F:structural molecule activity; IEA:InterPro.
DR   InterPro; IPR007586; VP8_pox_nuc-bd.
DR   Pfam; PF04498; Pox_VP8_L4R; 1.
PE   2: Evidence at transcript level;
KW   Late protein; Reference proteome; Virion.
FT   PROPEP          1..32
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000040593"
FT   CHAIN           33..251
FT                   /note="Core protein VP8"
FT                   /id="PRO_0000040594"
FT   SITE            18..19
FT                   /note="Cleavage; by core protease I7"
FT                   /evidence="ECO:0000250"
FT   SITE            32..33
FT                   /note="Cleavage; by core protease I7"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   251 AA;  28487 MW;  BA34201F43FBD5B1 CRC64;
     MSLLLENLIE EDTIFFAGSI SEYDDLQMVI AGAKSKFPRS MLSIFNIVPR TMSKYELELI
     HNENITGAMF TTMYNIRNNL GLGDDKLTIE AIENYFLDPN NEVMPLIINN TDMTAVIPKK
     SGRRKNKNMV IFRQGSSPIL CIFETRKKIN IYKENMESAS TEYTPIGDNK ALISKYAGIN
     VLNVYSPSTS MRLNAIYGFT NKNKLEKLST NKELELYSSS PLQEPIRLND FLGLLECVKK
     NIPLTDIPTK D