ID   VPA_BP186               Reviewed;         694 AA.
AC   P41064;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 2.
DT   02-JUN-2021, entry version 85.
DE   RecName: Full=Replication gene A protein;
DE            EC=3.1.-.-;
DE   AltName: Full=GpA;
GN   Name=A; Synonyms=CP87;
OS   Escherichia phage 186 (Bacteriophage 186).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Peduovirinae; Eganvirus.
OX   NCBI_TaxID=29252;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=186CITSP;
RX   PubMed=2352277; DOI=10.1016/s0022-2836(05)80207-4;
RA   Sivaprasad A.V., Jarvinen R., Puspurs A., Egan J.B.;
RT   "DNA replication studies with coliphage 186. III. A single phage gene is
RT   required for phage 186 replication.";
RL   J. Mol. Biol. 213:449-463(1990).
CC   -!- FUNCTION: Endonuclease which induces a single-strand cut at or near
CC       ori. May act by forming a covalent link to the 5'side of the nick.
CC   -!- SIMILARITY: Belongs to the phage GPA family. {ECO:0000305}.
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DR   EMBL; U32222; AAC34186.1; -; Genomic_DNA.
DR   PIR; S10632; S10632.
DR   RefSeq; NP_052289.1; NC_001317.1.
DR   PRIDE; P41064; -.
DR   GeneID; 1262442; -.
DR   KEGG; vg:1262442; -.
DR   Proteomes; UP000000369; Genome.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR   InterPro; IPR008766; Replication_gene_A.
DR   Pfam; PF05840; Phage_GPA; 1.
PE   3: Inferred from homology;
KW   Covalent protein-DNA linkage; DNA replication; Early protein; Endonuclease;
KW   Hydrolase; Nuclease; Reference proteome; Viral DNA replication.
FT   CHAIN           1..694
FT                   /note="Replication gene A protein"
FT                   /id="PRO_0000165297"
FT   REGION          605..659
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          672..694
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        608..623
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        641..655
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        672..686
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        442
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        446
FT                   /note="O-(5'-phospho-DNA)-tyrosine intermediate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   694 AA;  79580 MW;  28E311E7B43509B8 CRC64;
     MTGVVYAFPW NAPRSAIASS YLTYDQQHRR DRMFAALLHA RKVLFLQPEC VRFDVYRTAA
     VLEQNQGSQR ANAFLISFCK KALPRLELVA KKYECSGINS NVSAAVFDGH FDTQLMQYLA
     SRMVNMVARF NRLPDMSRAD IDLLAADIAN FIRAELADID DTGFSELKTL YTWYMRAGFI
     SLQFNVTPPK WERVTKKYFC EDEIAPAVMR MFNEVWWRGR LRRIAAAWRE HLQIAVGNVS
     KKRHAYASKN CVTDWREQKR RTREFLKGLD LEDEEGNRIS LIEKYDGSVA NPAIRRCELM
     ARIRGFENIC NELGYVGEFY TLTAPSKYHA TTKAGYRNSK WNGASPSDTQ SYLTGLWARI
     RAKLHREEIR IFGIRVAEPH HDGTPHWHML MFMLPEDVER VRLIIRDYAW EEDHYELRSD
     KAKKARFHAE AIDPEKGSAT GYVAKYISKN IDGYALDGET DDESGELLKE TAPAVSAWAA
     RWHIRQFQFI GGAPVTVYRE LRRMADPETA RALSVEFAAV HDAAHYGRWA DYVNAQGGPF
     VRRDDLQVRT LYEPRTEFNQ YGEETVCIKG VYDASIGAGS PILTRLTQWK IVPKRAVDLA
     VDVKGASAPS RSSVNNCTGS ESDPPILDLT KPLSRRERRE LTNRLRKKKP TTRRKFIHGT
     DKQNVAITKT IDEIHSDNRH HNQPGRSPAP DGRW