ID   VPB20_MYCTU             Reviewed;          73 AA.
AC   P9WJ45; L0TA46; P95003; Q7D6X8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 2.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Antitoxin VapB20;
GN   Name=vapB20; OrderedLocusNames=Rv2550c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   EXPRESSION IN E.COLI, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=19016878; DOI=10.1111/j.1574-6968.2008.01400.x;
RA   Gupta A.;
RT   "Killing activity and rescue function of genome-wide toxin-antitoxin loci
RT   of Mycobacterium tuberculosis.";
RL   FEMS Microbiol. Lett. 290:45-53(2009).
RN   [4]
RP   FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=24225902; DOI=10.1038/ncomms3796;
RA   Winther K.S., Brodersen D.E., Brown A.K., Gerdes K.;
RT   "VapC20 of Mycobacterium tuberculosis cleaves the sarcin-ricin loop of 23S
RT   rRNA.";
RL   Nat. Commun. 4:2796-2796(2013).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in E.coli neutralizes the toxic effect of cognate toxin
CC       VapC20. {ECO:0000269|PubMed:19016878, ECO:0000269|PubMed:24225902}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP45346.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305|PubMed:24225902};
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DR   EMBL; AL123456; CCP45346.1; ALT_INIT; Genomic_DNA.
DR   PIR; B70660; B70660.
DR   RefSeq; NP_217066.1; NC_000962.3.
DR   AlphaFoldDB; P9WJ45; -.
DR   SMR; P9WJ45; -.
DR   STRING; 83332.Rv2550c; -.
DR   PaxDb; P9WJ45; -.
DR   DNASU; 887353; -.
DR   GeneID; 887353; -.
DR   KEGG; mtu:Rv2550c; -.
DR   TubercuList; Rv2550c; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0097351; F:toxin sequestering activity; IPI:UniProtKB.
DR   GO; GO:0098754; P:detoxification; IMP:MTBBASE.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   InterPro; IPR002145; CopG.
DR   Pfam; PF01402; RHH_1; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..73
FT                   /note="Antitoxin VapB20"
FT                   /id="PRO_0000408066"
SQ   SEQUENCE   73 AA;  8199 MW;  979A21051AD7F8F2 CRC64;
     MKRLQIYIDE DVDRALAVEA RRRRTSKAAL IREYVAEHLR QPGPDPVDAF VGSFVGEADL
     SASVDDVVYG KHE