ID   VPB27_MYCTU             Reviewed;          78 AA.
AC   O07779; L0T6Z9;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 3.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Antitoxin VapB27;
GN   Name=vapB27; Synonyms=vapB-mt24; OrderedLocusNames=Rv0599c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   FUNCTION AS AN ANTITOXIN, SUBUNIT, AND INTERACTION WITH TOXINS MAZF6;
RP   MAZF9; VAPC27 AND VAPC40.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20876537; DOI=10.1074/jbc.m110.163105;
RA   Zhu L., Sharp J.D., Kobayashi H., Woychik N.A., Inouye M.;
RT   "Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and
RT   functionally interact.";
RL   J. Biol. Chem. 285:39732-39738(2010).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Cognate toxin is VapC27. Upon expression in E.coli partially
CC       counteracts the ribonuclease activity of non-cognate toxins MazF6 and
CC       MazF9. {ECO:0000269|PubMed:20876537}.
CC   -!- SUBUNIT: Interacts with cognate toxin VapC27 and non-cognate toxins
CC       MazF6 and VapC40. Interaction with MazF6 and MazF9 partially
CC       neutralizes the toxins. {ECO:0000269|PubMed:20876537}.
CC   -!- SIMILARITY: Belongs to the VapB family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP43338.1; -; Genomic_DNA.
DR   PIR; B70909; B70909.
DR   RefSeq; NP_215113.1; NC_000962.3.
DR   RefSeq; WP_003403139.1; NZ_NVQJ01000033.1.
DR   AlphaFoldDB; O07779; -.
DR   STRING; 83332.Rv0599c; -.
DR   PaxDb; O07779; -.
DR   DNASU; 887856; -.
DR   GeneID; 45424567; -.
DR   GeneID; 887856; -.
DR   KEGG; mtu:Rv0599c; -.
DR   TubercuList; Rv0599c; -.
DR   eggNOG; COG2002; Bacteria.
DR   InParanoid; O07779; -.
DR   OMA; TDEMMFA; -.
DR   PhylomeDB; O07779; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007159; SpoVT-AbrB_dom.
DR   InterPro; IPR037914; SpoVT-AbrB_sf.
DR   Pfam; PF04014; MazE_antitoxin; 1.
DR   SMART; SM00966; SpoVT_AbrB; 1.
DR   SUPFAM; SSF89447; SSF89447; 1.
DR   TIGRFAMs; TIGR01439; lp_hng_hel_AbrB; 1.
DR   PROSITE; PS51740; SPOVT_ABRB; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..78
FT                   /note="Antitoxin VapB27"
FT                   /id="PRO_0000408044"
FT   DOMAIN          1..45
FT                   /note="SpoVT-AbrB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
SQ   SEQUENCE   78 AA;  8212 MW;  10D8C631953A6B73 CRC64;
     MKAVVDAAGR IVVPKPLREA LGLQPGSTVE ISRYGAGLHL IPTGRTARLE EENGVLVATG
     ETTIDDEVVF GLIDSGRK