ID   VPB32_MYCTU             Reviewed;          65 AA.
AC   P9WJ33; L0T5Y0; O06565; Q7D8U3;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 29.
DE   RecName: Full=Antitoxin VapB32;
GN   Name=vapB32; OrderedLocusNames=Rv1113;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=23345537; DOI=10.1074/mcp.m112.018846;
RA   Albrethsen J., Agner J., Piersma S.R., Hoejrup P., Pham T.V., Weldingh K.,
RA   Jimenez C.R., Andersen P., Rosenkrands I.;
RT   "Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-
RT   starvation-responsive toxin-antitoxin systems.";
RL   Mol. Cell. Proteomics 12:1180-1191(2013).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in M.smegmatis neutralizes the effect of cognate toxin
CC       VapC32. {ECO:0000269|PubMed:20011113}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:23345537}.
CC       Note=Following 6 weeks of nutrient starvation (PubMed:23345537).
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DR   EMBL; AL123456; CCP43866.1; -; Genomic_DNA.
DR   PIR; B70537; B70537.
DR   RefSeq; NP_215629.1; NC_000962.3.
DR   RefSeq; WP_003405863.1; NZ_NVQJ01000021.1.
DR   AlphaFoldDB; P9WJ33; -.
DR   SMR; P9WJ33; -.
DR   STRING; 83332.Rv1113; -.
DR   PaxDb; P9WJ33; -.
DR   DNASU; 885450; -.
DR   GeneID; 45425087; -.
DR   GeneID; 885450; -.
DR   KEGG; mtu:Rv1113; -.
DR   TubercuList; Rv1113; -.
DR   eggNOG; COG5450; Bacteria.
DR   OMA; MDKADLF; -.
DR   PhylomeDB; P9WJ33; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0045927; P:positive regulation of growth; IMP:MTBBASE.
DR   InterPro; IPR019239; VapB_antitoxin.
DR   Pfam; PF09957; VapB_antitoxin; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Secreted; Toxin-antitoxin system.
FT   CHAIN           1..65
FT                   /note="Antitoxin VapB32"
FT                   /id="PRO_0000408076"
FT   REGION          46..65
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   65 AA;  6973 MW;  51000929DB16C82B CRC64;
     MRTTVTVDDA LLAKAAELTG VKEKSTLLRE GLQTLVRVES ARRLAALGGT DPQATAAPRR
     RTSPR