ID   VPB40_MYCTU             Reviewed;          81 AA.
AC   P9WFC3; L0TAC2; P65027; Q50626;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Antitoxin VapB40;
GN   Name=vapB40; Synonyms=vapB-mt25; OrderedLocusNames=Rv2595;
GN   ORFNames=MTCY227.06c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION AS AN ANTITOXIN, SUBUNIT, AND INTERACTION WITH TOXINS MAZF6; MAZF9
RP   AND VAPC40.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20876537; DOI=10.1074/jbc.m110.163105;
RA   Zhu L., Sharp J.D., Kobayashi H., Woychik N.A., Inouye M.;
RT   "Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and
RT   functionally interact.";
RL   J. Biol. Chem. 285:39732-39738(2010).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Its cognate toxin is VapC40. Upon expression in E.coli partially
CC       counteracts the ribonuclease activity of non-cognate toxins MazF6 and
CC       MazF9. {ECO:0000269|PubMed:20876537}.
CC   -!- SUBUNIT: Physically interacts with cognate toxin VapC40.
CC       {ECO:0000269|PubMed:20876537}.
CC   -!- SIMILARITY: Belongs to the VapB family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45391.1; -; Genomic_DNA.
DR   PIR; B70727; B70727.
DR   RefSeq; NP_217111.1; NC_000962.3.
DR   RefSeq; WP_003413429.1; NZ_NVQJ01000023.1.
DR   AlphaFoldDB; P9WFC3; -.
DR   SMR; P9WFC3; -.
DR   STRING; 83332.Rv2595; -.
DR   PaxDb; P9WFC3; -.
DR   DNASU; 887682; -.
DR   GeneID; 45426597; -.
DR   GeneID; 887682; -.
DR   KEGG; mtu:Rv2595; -.
DR   TubercuList; Rv2595; -.
DR   eggNOG; COG2002; Bacteria.
DR   PhylomeDB; P9WFC3; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR007159; SpoVT-AbrB_dom.
DR   InterPro; IPR037914; SpoVT-AbrB_sf.
DR   Pfam; PF04014; MazE_antitoxin; 1.
DR   SMART; SM00966; SpoVT_AbrB; 1.
DR   SUPFAM; SSF89447; SSF89447; 1.
DR   TIGRFAMs; TIGR01439; lp_hng_hel_AbrB; 1.
DR   PROSITE; PS51740; SPOVT_ABRB; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..81
FT                   /note="Antitoxin VapB40"
FT                   /id="PRO_0000104063"
FT   DOMAIN          1..45
FT                   /note="SpoVT-AbrB"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
SQ   SEQUENCE   81 AA;  9250 MW;  0DA5C2D8B50E8D6B CRC64;
     MRTTIDVAGR LVIPKRIRER LGLRGNDQVE ITERDGRIEI EPAPTGVELV REGSVLVARP
     ERPLPPLTDE IVRETLDRTR R