VPB45_MYCTU
ID VPB45_MYCTU Reviewed; 239 AA.
AC O53464; I6XZK1; L0TB40;
DT 27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 3.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Putative antitoxin VapB45 {ECO:0000303|PubMed:24662523};
GN Name=vapB45 {ECO:0000303|PubMed:24662523};
GN OrderedLocusNames=Rv2018, RVBD_2018; ORFNames=LH57_10995, P425_02089;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genome Sequencing Platform;
RA Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RG The Broad Institute Genomics Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RT "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RA Hazbon M.H., Riojas M.A., Damon A.M., Alalade R.O., Cantwell B.J.,
RA Monaco A., King S., Sohrabi A.;
RT "Phylogenetic analysis of Mycobacterial species using whole genome
RT sequences.";
RL Submitted (SEP-2014) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN [6]
RP GENE NAME, DISCUSSION OF FUNCTION, AND REVIEW.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=24662523; DOI=10.3390/toxins6031002;
RA Sala A., Bordes P., Genevaux P.;
RT "Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.";
RL Toxins 6:1002-1020(2014).
CC -!- FUNCTION: Possibly the antitoxin component of a type II toxin-antitoxin
CC (TA) system. Its cognate toxin is VapC45.
CC {ECO:0000305|PubMed:24662523}.
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DR EMBL; CP003248; AFN49958.1; -; Genomic_DNA.
DR EMBL; CP009480; AIR14768.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP44790.1; -; Genomic_DNA.
DR EMBL; JLDD01000023; KBJ33162.1; -; Genomic_DNA.
DR RefSeq; NP_216534.1; NC_000962.3.
DR RefSeq; WP_003410108.1; NZ_NVQJ01000046.1.
DR PDB; 5AF3; X-ray; 1.78 A; A/B=1-239.
DR PDBsum; 5AF3; -.
DR AlphaFoldDB; O53464; -.
DR SMR; O53464; -.
DR STRING; 83332.Rv2018; -.
DR PaxDb; O53464; -.
DR PRIDE; O53464; -.
DR DNASU; 887560; -.
DR GeneID; 887560; -.
DR KEGG; mtu:Rv2018; -.
DR KEGG; mtv:RVBD_2018; -.
DR PATRIC; fig|83332.111.peg.2249; -.
DR TubercuList; Rv2018; -.
DR eggNOG; COG2442; Bacteria.
DR HOGENOM; CLU_092327_0_0_11; -.
DR OMA; VHQVEPV; -.
DR PhylomeDB; O53464; -.
DR Proteomes; UP000001584; Chromosome.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR007367; DUF433.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017277; VapB45-like.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR Pfam; PF04255; DUF433; 1.
DR PIRSF; PIRSF037738; UCP037738; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..239
FT /note="Putative antitoxin VapB45"
FT /id="PRO_0000433058"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 18..25
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 29..36
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 66..68
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 70..84
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 93..103
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 113..128
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 133..141
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 142..145
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 148..151
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 153..159
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 162..164
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 168..174
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 176..181
FT /evidence="ECO:0007829|PDB:5AF3"
FT STRAND 183..185
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 190..192
FT /evidence="ECO:0007829|PDB:5AF3"
FT TURN 197..199
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 204..211
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 216..223
FT /evidence="ECO:0007829|PDB:5AF3"
FT HELIX 227..234
FT /evidence="ECO:0007829|PDB:5AF3"
SQ SEQUENCE 239 AA; 26034 MW; 8F823BA0821FD1DE CRC64;
MAGDQELELR FDVPLYTLAE ASRYLVVPRA TLATWADGYE RRPANAPAVQ GQPIITALPH
PTGSHARLPF VGIAEAYVLN AFRRAGVPMQ RIRPSLDWLI KNVGPHALAS QDLCTDGAEV
LWRFAERSGE GSPDDLVVRG LIVPRSGQYV FKEIVEHYLQ QISFADDNLA SMIRLPQYGD
ANVVLDPRRG YGQPVFDGSG VRVADVLGPL RAGATFQAVA DDYGVTPDQL RDALDAIAA
