ID   VPB46_MYCTU             Reviewed;         102 AA.
AC   P9WF13; L0TF80; O50412; Q7D5L3;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 35.
DE   RecName: Full=Antitoxin VapB46;
GN   Name=vapB46; OrderedLocusNames=Rv3385c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS AN ANTITOXIN.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system.
CC       Upon expression in M.smegmatis neutralizes the effect of cognate toxin
CC       VapC46. {ECO:0000269|PubMed:20011113}.
CC   -!- SIMILARITY: Belongs to the phD/YefM antitoxin family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP46206.1; -; Genomic_DNA.
DR   PIR; H70973; H70973.
DR   RefSeq; NP_217902.1; NC_000962.3.
DR   RefSeq; WP_003417918.1; NC_018143.2.
DR   AlphaFoldDB; P9WF13; -.
DR   SMR; P9WF13; -.
DR   STRING; 83332.Rv3385c; -.
DR   PaxDb; P9WF13; -.
DR   DNASU; 887429; -.
DR   GeneID; 887429; -.
DR   KEGG; mtu:Rv3385c; -.
DR   PATRIC; fig|83332.111.peg.3773; -.
DR   TubercuList; Rv3385c; -.
DR   eggNOG; COG4118; Bacteria.
DR   OMA; RSTDEWL; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0097351; F:toxin sequestering activity; IBA:GO_Central.
DR   GO; GO:0045927; P:positive regulation of growth; IMP:MTBBASE.
DR   InterPro; IPR006442; Antitoxin_Phd/YefM.
DR   InterPro; IPR036165; YefM-like_sf.
DR   Pfam; PF02604; PhdYeFM_antitox; 1.
DR   SUPFAM; SSF143120; SSF143120; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..102
FT                   /note="Antitoxin VapB46"
FT                   /id="PRO_0000408050"
SQ   SEQUENCE   102 AA;  11031 MW;  644D4A89C5C2F79D CRC64;
     MTPTACATVS TMTSVGVRAL RQRASELLRR VEAGETIEIT DRGRPVALLS PLPQGGPYEQ
     LLASGEIERA TLDVVDLPEP LDLDAGVELP SVTLARLREH ER