ID   VPB49_MYCTU             Reviewed;         150 AA.
AC   P9WF15; L0TBS0; O53331; Q7D5Z5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   25-MAY-2022, entry version 32.
DE   RecName: Full=Putative antitoxin VapB49 {ECO:0000303|PubMed:24662523};
GN   Name=vapB49 {ECO:0000303|PubMed:24662523}; OrderedLocusNames=Rv3181c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   INDUCTION IN PERSISTER CELLS.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21673191; DOI=10.1128/mbio.00100-11;
RA   Keren I., Minami S., Rubin E., Lewis K.;
RT   "Characterization and transcriptome analysis of Mycobacterium tuberculosis
RT   persisters.";
RL   MBio 2:E00100-E00111(2011).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [5]
RP   GENE NAME, DISCUSSION OF FUNCTION, AND REVIEW.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=24662523; DOI=10.3390/toxins6031002;
RA   Sala A., Bordes P., Genevaux P.;
RT   "Multiple toxin-antitoxin systems in Mycobacterium tuberculosis.";
RL   Toxins 6:1002-1020(2014).
CC   -!- FUNCTION: Possibly the antitoxin component of a type II toxin-antitoxin
CC       (TA) system. Its cognate toxin is VapC49 (Potential). {ECO:0000305}.
CC   -!- INDUCTION: Induced in persister cells in response to D-cycloserine.
CC       {ECO:0000269|PubMed:21673191}.
CC   -!- SIMILARITY: Belongs to the phD/YefM antitoxin family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AL123456; CCP45992.1; -; Genomic_DNA.
DR   PIR; C70949; C70949.
DR   RefSeq; NP_217697.1; NC_000962.3.
DR   RefSeq; WP_003899953.1; NZ_NVQJ01000019.1.
DR   AlphaFoldDB; P9WF15; -.
DR   SMR; P9WF15; -.
DR   STRING; 83332.Rv3181c; -.
DR   PaxDb; P9WF15; -.
DR   DNASU; 888787; -.
DR   GeneID; 45427170; -.
DR   GeneID; 888787; -.
DR   KEGG; mtu:Rv3181c; -.
DR   TubercuList; Rv3181c; -.
DR   eggNOG; COG4118; Bacteria.
DR   OMA; SHRDIDR; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0097351; F:toxin sequestering activity; IBA:GO_Central.
DR   InterPro; IPR006442; Antitoxin_Phd/YefM.
DR   InterPro; IPR036165; YefM-like_sf.
DR   Pfam; PF02604; PhdYeFM_antitox; 1.
DR   SUPFAM; SSF143120; SSF143120; 1.
PE   1: Evidence at protein level;
KW   Reference proteome; Toxin-antitoxin system.
FT   CHAIN           1..150
FT                   /note="Putative antitoxin VapB49"
FT                   /id="PRO_0000408049"
FT   REGION          124..150
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   150 AA;  16799 MW;  E987C6E7708019C5 CRC64;
     MQLGRKVTSH HDIDRFGVAS TADESVYRPL PPRLRLAQVN LSRRRCRTQS DMYKSRFSEC
     TVQSVDVSVT ELRAHLSDWL DRARAGGEVV ITERGIPIAR LAALDSTDTL ERLTAEGVIG
     KATAQRPVAA GRPRPRPQRP VSDRVSDQRR