ID   VPC13_MYCTU             Reviewed;         131 AA.
AC   P9WFA1; L0TAR6; P64901; P95168; Q50595;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Ribonuclease VapC13 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC13 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC13 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC13 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=Rv1838c;
GN   ORFNames=MTCY1A11.05, MTCY359.35;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 27294 / TMC 102 / H37Rv;
RX   PubMed=23345537; DOI=10.1074/mcp.m112.018846;
RA   Albrethsen J., Agner J., Piersma S.R., Hoejrup P., Pham T.V., Weldingh K.,
RA   Jimenez C.R., Andersen P., Rosenkrands I.;
RT   "Proteomic profiling of Mycobacterium tuberculosis identifies nutrient-
RT   starvation-responsive toxin-antitoxin systems.";
RL   Mol. Cell. Proteomics 12:1180-1191(2013).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB13 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SUBCELLULAR LOCATION: Secreted. Note=Following 6 weeks of nutrient
CC       starvation. {ECO:0000269|PubMed:23345537}.
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AL123456; CCP44604.1; -; Genomic_DNA.
DR   PIR; F70663; F70663.
DR   RefSeq; NP_216354.1; NC_000962.3.
DR   RefSeq; WP_003409273.1; NZ_NVQJ01000013.1.
DR   AlphaFoldDB; P9WFA1; -.
DR   SMR; P9WFA1; -.
DR   STRING; 83332.Rv1838c; -.
DR   PaxDb; P9WFA1; -.
DR   DNASU; 885744; -.
DR   GeneID; 885744; -.
DR   KEGG; mtu:Rv1838c; -.
DR   TubercuList; Rv1838c; -.
DR   eggNOG; COG1848; Bacteria.
DR   OMA; MARHHIT; -.
DR   PhylomeDB; P9WFA1; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Secreted; Toxin-antitoxin system.
FT   CHAIN           1..131
FT                   /note="Ribonuclease VapC13"
FT                   /id="PRO_0000103911"
FT   DOMAIN          2..128
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         99
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   131 AA;  14726 MW;  C164346E951BFE7E CRC64;
     MILVDSNIPM YLVGASHPHK LDAQRLLESA LSGGERLVTD AEVLQEICHR YVAIKRREAI
     QPAFDAIIGV VDEVLPIERT DVEHARDALL RYQTLSARDA LHIAVMAHHD ITRLMSFDRG
     FDSYPGIKRL A