ID   VPC14_MYCTO             Reviewed;         103 AA.
AC   P9WF98; L0TAW9; P95261; Q7D7Q0;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Ribonuclease VapC14;
DE            Short=RNase VapC14;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin VapC14;
GN   Name=vapC14; OrderedLocusNames=MT2003;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB14 (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK46275.1; -; Genomic_DNA.
DR   PIR; E70638; E70638.
DR   RefSeq; WP_003409881.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF98; -.
DR   EnsemblBacteria; AAK46275; AAK46275; MT2003.
DR   GeneID; 45425924; -.
DR   KEGG; mtc:MT2003; -.
DR   PATRIC; fig|83331.31.peg.2158; -.
DR   HOGENOM; CLU_178458_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..103
FT                   /note="Ribonuclease VapC14"
FT                   /id="PRO_0000428576"
FT   DOMAIN          3..74
FT                   /note="PINc"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   103 AA;  11628 MW;  CEFCDA46EB85C635 CRC64;
     MTYVLDTNVV SALRVPGRHP AVAAWADSVQ VAEQFVVAIT LAEIERGVIA KERTDPTQSE
     HLRRWFDDKV LRIFVFARRG TNLIMQPLAG HIGYSLYSGI SWF