ID   VPC14_MYCTU             Reviewed;         103 AA.
AC   P9WF99; L0TAW9; P95261; Q7D7Q0;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 34.
DE   RecName: Full=Ribonuclease VapC14;
DE            Short=RNase VapC14;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin VapC14;
GN   Name=vapC14; OrderedLocusNames=Rv1953;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=15718296; DOI=10.1093/nar/gki201;
RA   Pandey D.P., Gerdes K.;
RT   "Toxin-antitoxin loci are highly abundant in free-living but lost from
RT   host-associated prokaryotes.";
RL   Nucleic Acids Res. 33:966-976(2005).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. The cognate antitoxin is VapB14 (By similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP44720.1; -; Genomic_DNA.
DR   PIR; E70638; E70638.
DR   RefSeq; NP_216469.1; NC_000962.3.
DR   RefSeq; WP_003409881.1; NZ_NVQJ01000048.1.
DR   AlphaFoldDB; P9WF99; -.
DR   STRING; 83332.Rv1953; -.
DR   PaxDb; P9WF99; -.
DR   DNASU; 885970; -.
DR   GeneID; 45425924; -.
DR   GeneID; 885970; -.
DR   KEGG; mtu:Rv1953; -.
DR   TubercuList; Rv1953; -.
DR   eggNOG; COG1487; Bacteria.
DR   PhylomeDB; P9WF99; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004518; F:nuclease activity; IEA:UniProtKB-KW.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..103
FT                   /note="Ribonuclease VapC14"
FT                   /id="PRO_0000407874"
FT   DOMAIN          3..74
FT                   /note="PINc"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   103 AA;  11628 MW;  CEFCDA46EB85C635 CRC64;
     MTYVLDTNVV SALRVPGRHP AVAAWADSVQ VAEQFVVAIT LAEIERGVIA KERTDPTQSE
     HLRRWFDDKV LRIFVFARRG TNLIMQPLAG HIGYSLYSGI SWF