ID   VPC19_MYCTO             Reviewed;         125 AA.
AC   P9WF92; L0TCP7; P95005; Q7D6X9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Ribonuclease VapC19 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC19 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC19 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC19 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT2624;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its toxic effect is neutralized by coexpression with cognate
CC       antitoxin VapB19 (By similarity). {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; AAK46935.1; -; Genomic_DNA.
DR   PIR; H70659; H70659.
DR   RefSeq; WP_003413174.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF92; -.
DR   SMR; P9WF92; -.
DR   EnsemblBacteria; AAK46935; AAK46935; MT2624.
DR   GeneID; 45426550; -.
DR   KEGG; mtc:MT2624; -.
DR   PATRIC; fig|83331.31.peg.2830; -.
DR   HOGENOM; CLU_118482_0_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..125
FT                   /note="Ribonuclease VapC19"
FT                   /id="PRO_0000428579"
FT   DOMAIN          3..122
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         93
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   125 AA;  13761 MW;  289DFBE3F8C8A93D CRC64;
     MKLIDTTIAV DHLRGEPAAA VLLAELINNG EEIAASELVR FELLAGVRES ELAALEAFFS
     AVVWTLVTED IARIGGRLAR RYRSSHRGID DVDYLIAATA IVVDADLLTT NVRHFPMFPD
     LQPPY