ID   VPC20_MYCTO             Reviewed;         131 AA.
AC   P0DMV7;
DT   27-MAY-2015, integrated into UniProtKB/Swiss-Prot.
DT   27-MAY-2015, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=23S rRNA-specific endonuclease VapC20 {ECO:0000305};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Ribonuclease VapC20 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC20 {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC20 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC20; OrderedLocusNames=MT2626.1;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       endoribonuclease that cleaves 23S rRNA in the sarcin-ricin loop (SRL).
CC       The SRL sequence is highly conserved and is implicated in GTP
CC       hydrolysis by EF-Tu and EF-G. Acts on purified ribosomes but not on
CC       isolated RNA. Its toxic effect is neutralized by coexpression with
CC       cognate antitoxin VapB20. {ECO:0000250|UniProtKB:P95004,
CC       ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_003413180.1; NZ_KK341227.1.
DR   AlphaFoldDB; P0DMV7; -.
DR   SMR; P0DMV7; -.
DR   PATRIC; fig|83331.31.peg.2832; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0004521; F:endoribonuclease activity; IEA:InterPro.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   CDD; cd18680; PIN_MtVapC20-like; 1.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR039018; VapC20.
DR   InterPro; IPR022907; VapC_family.
DR   PANTHER; PTHR42188; PTHR42188; 1.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Endonuclease; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW   Toxin-antitoxin system.
FT   CHAIN           1..131
FT                   /note="23S rRNA-specific endonuclease VapC20"
FT                   /id="PRO_0000433055"
FT   DOMAIN          2..125
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         98
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   131 AA;  14620 MW;  1AF9B15EF70E96A7 CRC64;
     MIFVDTSFWA ALGNAGDARH GTAKRLWASK PPVVMTSNHV LGETWTLLNR RCGHRAAVAA
     AAIRLSTVVR VEHVTADLEE QAWEWLVRHD EREYSFVDAT SFAVMRKKGI QNAYAFDGDF
     SAAGFVEVRP E