VPC26_MYCTU
ID VPC26_MYCTU Reviewed; 135 AA.
AC O53779; L0T439;
DT 03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Ribonuclease VapC26 {ECO:0000255|HAMAP-Rule:MF_00265};
DE Short=RNase VapC26 {ECO:0000255|HAMAP-Rule:MF_00265};
DE EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE AltName: Full=Toxin VapC26 {ECO:0000255|HAMAP-Rule:MF_00265};
GN Name=vapC26 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=Rv0582;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP EXPRESSION IN M.SMEGMATIS, AND FUNCTION AS A TOXIN.
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA Ramage H.R., Connolly L.E., Cox J.S.;
RT "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT antitoxin systems: implications for pathogenesis, stress responses, and
RT evolution.";
RL PLoS Genet. 5:E1000767-E1000767(2009).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC RNase (By similarity). Upon expression in M.smegmatis inhibits colony
CC formation. Its toxic effect is neutralized by coexpression with cognate
CC antitoxin VapB26. {ECO:0000255|HAMAP-Rule:MF_00265,
CC ECO:0000269|PubMed:20011113}.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR EMBL; AL123456; CCP43320.1; -; Genomic_DNA.
DR PIR; E70934; E70934.
DR RefSeq; NP_215096.1; NC_000962.3.
DR RefSeq; WP_003403047.1; NZ_NVQJ01000033.1.
DR PDB; 5X3T; X-ray; 2.65 A; B/D/F/H=1-135.
DR PDBsum; 5X3T; -.
DR AlphaFoldDB; O53779; -.
DR SMR; O53779; -.
DR STRING; 83332.Rv0582; -.
DR PaxDb; O53779; -.
DR DNASU; 887747; -.
DR GeneID; 887747; -.
DR KEGG; mtu:Rv0582; -.
DR TubercuList; Rv0582; -.
DR eggNOG; COG2402; Bacteria.
DR InParanoid; O53779; -.
DR OMA; QSVRVRY; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR GO; GO:0045926; P:negative regulation of growth; IMP:MTBBASE.
DR HAMAP; MF_00265; VapC_Nob1; 1.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR002716; PIN_dom.
DR InterPro; IPR022907; VapC_family.
DR Pfam; PF01850; PIN; 1.
DR SUPFAM; SSF88723; SSF88723; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Hydrolase; Magnesium; Metal-binding; Nuclease;
KW Reference proteome; Toxin-antitoxin system.
FT CHAIN 1..135
FT /note="Ribonuclease VapC26"
FT /id="PRO_0000407885"
FT DOMAIN 1..118
FT /note="PINc"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 4
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT BINDING 97
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT STRAND 1..3
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 5..12
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 19..26
FT /evidence="ECO:0007829|PDB:5X3T"
FT STRAND 33..35
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 37..50
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 53..64
FT /evidence="ECO:0007829|PDB:5X3T"
FT STRAND 65..70
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 75..87
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 89..91
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 94..106
FT /evidence="ECO:0007829|PDB:5X3T"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:5X3T"
FT HELIX 117..121
FT /evidence="ECO:0007829|PDB:5X3T"
FT STRAND 132..134
FT /evidence="ECO:0007829|PDB:5X3T"
SQ SEQUENCE 135 AA; 14503 MW; E41EEBD26C0D28E9 CRC64;
MIIDTSALLA YFDAAEPDHA AVSECIDSSA DALVVSPYVV AELDYLVATR VGVDAELAVL
RELAGGAWEL ANCGAAEIEQ AARIVTKYQD QRIGIADAAN VVLADRYRTR TILTLDRRHF
SALRPIGGGR FTVIP
