ID   VPC27_MYCTO             Reviewed;         137 AA.
AC   P9WF82; L0T753; O07780; Q7D9K3;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Ribonuclease VapC27 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC27 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC27 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC27 {ECO:0000255|HAMAP-Rule:MF_00265}; Synonyms=vapC-mt24;
GN   OrderedLocusNames=MT0628;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Probably the toxic component of a type II toxin-antitoxin
CC       (TA) system. An RNase. Its cognate antitoxin is VapB27 (By similarity).
CC       {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- CAUTION: Bioinformatics programs predicts this could have a signal
CC       sequence. {ECO:0000305}.
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DR   EMBL; AE000516; AAK44852.1; -; Genomic_DNA.
DR   PIR; A70909; A70909.
DR   RefSeq; WP_003403137.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF82; -.
DR   SMR; P9WF82; -.
DR   EnsemblBacteria; AAK44852; AAK44852; MT0628.
DR   GeneID; 45424566; -.
DR   KEGG; mtc:MT0628; -.
DR   PATRIC; fig|83331.31.peg.661; -.
DR   HOGENOM; CLU_150004_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..137
FT                   /note="Ribonuclease VapC27"
FT                   /id="PRO_0000428584"
FT   DOMAIN          7..125
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         101
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   137 AA;  14818 MW;  12975B4555CA4402 CRC64;
     MKPPLAVDTS VAIPLLVRTH TAHAAVVAWW AHREAALCGH ALAETYSVLT RLPRDLRLAP
     MDAARLLTER FAAPLLLSSR TTEHLPRVLA QFEITGGAVY DALVALAAAE HRAELATRDA
     RAKDTYEKIG VHVVVAA