ID   VPC29_MYCTU             Reviewed;         133 AA.
AC   P9WF79; L0T729; O07760; Q7D9I9;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Ribonuclease VapC29 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC29 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC29 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC29 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=Rv0617;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [4]
RP   FUNCTION, ACTIVITY REGULATION, AND COFACTOR.
RX   PubMed=22539524; DOI=10.1261/rna.031229.111;
RA   McKenzie J.L., Duyvestyn J.M., Smith T., Bendak K., Mackay J., Cursons R.,
RA   Cook G.M., Arcus V.L.;
RT   "Determination of ribonuclease sequence-specificity using Pentaprobes and
RT   mass spectrometry.";
RL   RNA 18:1267-1278(2012).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. Its
CC       cognate antitoxin is VapB29 (By similarity). Has ribonuclease activity.
CC       {ECO:0000250, ECO:0000269|PubMed:22539524}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265,
CC         ECO:0000269|PubMed:22539524};
CC   -!- ACTIVITY REGULATION: Inhibited by EDTA. {ECO:0000269|PubMed:22539524}.
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AL123456; CCP43358.1; -; Genomic_DNA.
DR   PIR; D70911; D70911.
DR   RefSeq; NP_215131.1; NC_000962.3.
DR   RefSeq; WP_003403218.1; NZ_NVQJ01000033.1.
DR   AlphaFoldDB; P9WF79; -.
DR   SMR; P9WF79; -.
DR   STRING; 83332.Rv0617; -.
DR   PaxDb; P9WF79; -.
DR   GeneID; 45424579; -.
DR   GeneID; 887926; -.
DR   KEGG; mtu:Rv0617; -.
DR   TubercuList; Rv0617; -.
DR   eggNOG; COG1848; Bacteria.
DR   OMA; ARTHNGR; -.
DR   PhylomeDB; P9WF79; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:UniProt.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR006226; Mtu_PIN.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00028; Mtu_PIN_fam; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..133
FT                   /note="Ribonuclease VapC29"
FT                   /id="PRO_0000407887"
FT   DOMAIN          3..122
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         97
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   133 AA;  13970 MW;  98B9993106CF372D CRC64;
     MTVLLDANVL IALVVAEHVH HDAAADWLMA SDTGFATCPM TQGSLVRFLV RSGQSAAAAR
     DVVSAVQCTS RHEFWPDALS FAGVEVAGVV GHRQVTDAYL AQLARSHDGQ LATLDSGLAH
     LHGDVAVLIP TTT