ID   VPC39_MYCTO             Reviewed;         139 AA.
AC   P9WF62; L0TBH2; P95023; Q7D6Y8;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 40.
DE   RecName: Full=Ribonuclease VapC39 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC39 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC39 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC39 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT2605;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its toxic effect is neutralized by coexpression with cognate
CC       antitoxin VapB39 (By similarity). {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; AAK46915.1; -; Genomic_DNA.
DR   PIR; F70657; F70657.
DR   RefSeq; WP_003412970.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF62; -.
DR   SMR; P9WF62; -.
DR   EnsemblBacteria; AAK46915; AAK46915; MT2605.
DR   GeneID; 45426530; -.
DR   KEGG; mtc:MT2605; -.
DR   PATRIC; fig|83331.31.peg.2810; -.
DR   HOGENOM; CLU_145365_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:UniProt.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR006226; Mtu_PIN.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00028; Mtu_PIN_fam; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..139
FT                   /note="Ribonuclease VapC39"
FT                   /id="PRO_0000428594"
FT   DOMAIN          4..133
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         106
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   139 AA;  14765 MW;  47B4B1ADBD0870C6 CRC64;
     MTALLDVNVL IALGWPNHVH HAAAQRWFTQ FSSNGWATTP ITEAGYVRIS SNRSVMQVST
     TPAIAIAQLA AMTSLAGHTF WPDDVPLIVG SAGDRDAVSN HRRVTDCHLI ALAARYGGRL
     VTFDAALADS ASAGLVEVL