ID   VPC40_MYCTO             Reviewed;         134 AA.
AC   P9WF60; L0TD12; Q50625;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Ribonuclease VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC40 {ECO:0000255|HAMAP-Rule:MF_00265}; Synonyms=vapC-mt25;
GN   OrderedLocusNames=MT2672;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its cognate antitoxin is VapB40 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- CAUTION: Bioinformatics programs predicts this could have a signal
CC       sequence. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK46986.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK46986.1; ALT_INIT; Genomic_DNA.
DR   PIR; C70727; C70727.
DR   RefSeq; WP_003899389.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF60; -.
DR   SMR; P9WF60; -.
DR   EnsemblBacteria; AAK46986; AAK46986; MT2672.
DR   KEGG; mtc:MT2672; -.
DR   PATRIC; fig|83331.31.peg.2880; -.
DR   HOGENOM; CLU_150004_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..134
FT                   /note="Ribonuclease VapC40"
FT                   /id="PRO_0000428595"
FT   DOMAIN          3..126
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         98
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   134 AA;  14414 MW;  03D18E243430A67E CRC64;
     MIAPDTSVLV AGFATWHEGH EAAVRALNRG VHLIAHAAVE TYSVLTRLPP PHRIAPVAVH
     AYLADITSSN YLALDACSYR GLTDHLAEHD VTGGATYDAL VGFTAKAAGA KLLTRDLRAV
     ETYERLRVEV ELVT