ID   VPC40_MYCTU             Reviewed;         134 AA.
AC   P9WF61; L0TD12; Q50625;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Ribonuclease VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC40 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC40 {ECO:0000255|HAMAP-Rule:MF_00265}; Synonyms=vapC-mt25;
GN   OrderedLocusNames=Rv2596; ORFNames=MTCY227.05c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   INTERACTION WITH ANTITOXINS VAPB27 AND VAPB40.
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=20876537; DOI=10.1074/jbc.m110.163105;
RA   Zhu L., Sharp J.D., Kobayashi H., Woychik N.A., Inouye M.;
RT   "Noncognate Mycobacterium tuberculosis toxin-antitoxins can physically and
RT   functionally interact.";
RL   J. Biol. Chem. 285:39732-39738(2010).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase (By similarity). Its cognate antitoxin is VapB40.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SUBUNIT: Physically interacts with non-cognate antitoxin VapB27 and
CC       cognate antitoxin VapB40. {ECO:0000269|PubMed:20876537}.
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- CAUTION: Bioinformatics programs predicts this could have a signal
CC       sequence. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45392.1; -; Genomic_DNA.
DR   PIR; C70727; C70727.
DR   RefSeq; NP_217112.1; NC_000962.3.
DR   RefSeq; WP_003899389.1; NZ_NVQJ01000023.1.
DR   AlphaFoldDB; P9WF61; -.
DR   SMR; P9WF61; -.
DR   STRING; 83332.Rv2596; -.
DR   PaxDb; P9WF61; -.
DR   DNASU; 888218; -.
DR   GeneID; 888218; -.
DR   KEGG; mtu:Rv2596; -.
DR   TubercuList; Rv2596; -.
DR   eggNOG; COG1848; Bacteria.
DR   OMA; WHEGHES; -.
DR   PhylomeDB; P9WF61; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..134
FT                   /note="Ribonuclease VapC40"
FT                   /id="PRO_0000014142"
FT   DOMAIN          3..126
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         98
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   134 AA;  14414 MW;  03D18E243430A67E CRC64;
     MIAPDTSVLV AGFATWHEGH EAAVRALNRG VHLIAHAAVE TYSVLTRLPP PHRIAPVAVH
     AYLADITSSN YLALDACSYR GLTDHLAEHD VTGGATYDAL VGFTAKAAGA KLLTRDLRAV
     ETYERLRVEV ELVT