ID   VPC41_MYCTO             Reviewed;         146 AA.
AC   P9WF58; L0TBR4; O33215; Q7D6X2;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Ribonuclease VapC41 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC41 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC41 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC41 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT2677;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its toxic effect is neutralized by coexpression with cognate
CC       antitoxin VapB41 (By similarity). {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AE000516; AAK46993.1; -; Genomic_DNA.
DR   PIR; E70500; E70500.
DR   RefSeq; WP_003413460.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF58; -.
DR   SMR; P9WF58; -.
DR   EnsemblBacteria; AAK46993; AAK46993; MT2677.
DR   GeneID; 45426605; -.
DR   KEGG; mtc:MT2677; -.
DR   PATRIC; fig|83331.31.peg.2887; -.
DR   HOGENOM; CLU_146668_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:UniProt.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR006226; Mtu_PIN.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00028; Mtu_PIN_fam; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..146
FT                   /note="Ribonuclease VapC41"
FT                   /id="PRO_0000428596"
FT   DOMAIN          3..142
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         112
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   146 AA;  16026 MW;  DB4763AD9065A933 CRC64;
     MLLCDTNIWL ALALSGHVHH RASRAWLDTI NAPGVIHFCR ATQQSLLRLL TNRTVLGAYG
     SPPLTNREAW AAYAAFLDDD RIVLAGAEPD GLEAQWRAFA VRQSPAPKVW MDAYLAAFAL
     TGGFELVTTD TAFTQYGGIE LRLLAK