ID   VPC47_MYCTO             Reviewed;         136 AA.
AC   P9WF48; L0TFI7; Q50717;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Ribonuclease VapC47 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC47 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC47 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC47 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT3516;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its toxic effect on colony formation is neutralized by
CC       coexpression with cognate antitoxin VapB47 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47854.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK47854.1; ALT_INIT; Genomic_DNA.
DR   PIR; E70736; E70736.
DR   RefSeq; WP_003417998.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF48; -.
DR   SMR; P9WF48; -.
DR   EnsemblBacteria; AAK47854; AAK47854; MT3516.
DR   GeneID; 45427404; -.
DR   KEGG; mtc:MT3516; -.
DR   PATRIC; fig|83331.31.peg.3774; -.
DR   HOGENOM; CLU_119496_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..136
FT                   /note="Ribonuclease VapC47"
FT                   /id="PRO_0000428601"
FT   DOMAIN          2..104
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         5
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         94
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   136 AA;  14728 MW;  0BED0252F1A06E2A CRC64;
     MIYMDTSALT KLLISEPETT ELRTWLTAQS GQGEDAATST LGRVELMRVV ARYGQPGQTE
     RARYLLDGLD ILPLTEPVIG LAETIGPATL RSLDAIHLAA AAQIKRELTA FVTYDHRLLS
     GCREVGFVTA SPGAVR