ID   VPC48_MYCTO             Reviewed;         145 AA.
AC   P9WF46; L0TG87; O69665; Q7D518;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Ribonuclease VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC48 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=MT3799;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its cognate antitoxin is VapB48 (By similarity).
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE000516; AAK48166.1; -; Genomic_DNA.
DR   PIR; B70793; B70793.
DR   RefSeq; WP_003899644.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF46; -.
DR   SMR; P9WF46; -.
DR   EnsemblBacteria; AAK48166; AAK48166; MT3799.
DR   KEGG; mtc:MT3799; -.
DR   PATRIC; fig|83331.31.peg.4091; -.
DR   HOGENOM; CLU_146668_1_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:UniProt.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR006226; Mtu_PIN.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00028; Mtu_PIN_fam; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Toxin-antitoxin system.
FT   CHAIN           1..145
FT                   /note="Ribonuclease VapC48"
FT                   /id="PRO_0000428602"
FT   DOMAIN          15..141
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         109
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   145 AA;  16345 MW;  782434CF77DF552F CRC64;
     MSETFDVDVL VHATHRASPF HDKAKTLVER FLAGPGLVYL LWPVALGYLR VVTHPTLLGA
     PLAPEVAVEN IEQFTSRPHV RQVGEANGFW PVYRRVADPV KPRGNLVPDA HLVALMRHHG
     IATIWSHDRD FRKFEGIRIR DPFSG