ID   VPC48_MYCTU             Reviewed;         145 AA.
AC   P9WF47; L0TG87; O69665; Q7D518;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 38.
DE   RecName: Full=Ribonuclease VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            Short=RNase VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_00265};
DE   AltName: Full=Toxin VapC48 {ECO:0000255|HAMAP-Rule:MF_00265};
GN   Name=vapC48 {ECO:0000255|HAMAP-Rule:MF_00265}; OrderedLocusNames=Rv3697c;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   POSSIBLE FUNCTION.
RC   STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX   PubMed=20011113; DOI=10.1371/journal.pgen.1000767;
RA   Ramage H.R., Connolly L.E., Cox J.S.;
RT   "Comprehensive functional analysis of Mycobacterium tuberculosis toxin-
RT   antitoxin systems: implications for pathogenesis, stress responses, and
RT   evolution.";
RL   PLoS Genet. 5:E1000767-E1000767(2009).
RN   [3]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its cognate antitoxin is VapB48. {ECO:0000255|HAMAP-
CC       Rule:MF_00265, ECO:0000305|PubMed:20011113}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00265};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_00265}.
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DR   EMBL; AL123456; CCP46521.1; -; Genomic_DNA.
DR   PIR; B70793; B70793.
DR   RefSeq; NP_218214.1; NC_000962.3.
DR   RefSeq; WP_003899644.1; NZ_NVQJ01000028.1.
DR   AlphaFoldDB; P9WF47; -.
DR   SMR; P9WF47; -.
DR   STRING; 83332.Rv3697c; -.
DR   PaxDb; P9WF47; -.
DR   DNASU; 885492; -.
DR   GeneID; 885492; -.
DR   KEGG; mtu:Rv3697c; -.
DR   PATRIC; fig|83332.111.peg.4111; -.
DR   TubercuList; Rv3697c; -.
DR   eggNOG; COG1848; Bacteria.
DR   PhylomeDB; P9WF47; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   GO; GO:0045926; P:negative regulation of growth; IEA:UniProt.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR006226; Mtu_PIN.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
DR   TIGRFAMs; TIGR00028; Mtu_PIN_fam; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..145
FT                   /note="Ribonuclease VapC48"
FT                   /id="PRO_0000407899"
FT   DOMAIN          15..141
FT                   /note="PINc"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         6
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
FT   BINDING         109
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00265"
SQ   SEQUENCE   145 AA;  16345 MW;  782434CF77DF552F CRC64;
     MSETFDVDVL VHATHRASPF HDKAKTLVER FLAGPGLVYL LWPVALGYLR VVTHPTLLGA
     PLAPEVAVEN IEQFTSRPHV RQVGEANGFW PVYRRVADPV KPRGNLVPDA HLVALMRHHG
     IATIWSHDRD FRKFEGIRIR DPFSG