ID   VPC51_MYCTU             Reviewed;         137 AA.
AC   L0T5V6; I6Y770;
DT   03-SEP-2014, integrated into UniProtKB/Swiss-Prot.
DT   03-SEP-2014, sequence version 2.
DT   03-AUG-2022, entry version 36.
DE   RecName: Full=Ribonuclease VapC51 {ECO:0000305};
DE            Short=RNase VapC51;
DE            EC=3.1.-.-;
DE   AltName: Full=Toxin VapC51;
GN   Name=vapC51 {ECO:0000305}; OrderedLocusNames=Rv0229c, RVBD_0229c;
GN   ORFNames=P425_00238;
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genome Sequencing Platform;
RA   Galagan J., Kreiswirth B., Dobos K., Fortune S., Fitzgerald M., Young S.K.,
RA   Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Berlin A.M., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Gnerre S., Griggs A., Gujja S., Hansen M.,
RA   Howarth C., Imamovic A., Larimer J., McCowan C., Murphy C., Pearson M.,
RA   Poon T., Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J.,
RA   Nusbaum C., Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RG   The Broad Institute Genomics Platform;
RG   The Broad Institute Genome Sequencing Center for Infectious Disease;
RA   Earl A.M., Kreiswirth B., Gomez J., Victor T., Desjardins C., Abeel T.,
RA   Young S., Zeng Q., Gargeya S., Abouelleil A., Alvarado L., Chapman S.B.,
RA   Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C.,
RA   Imamovic A., Larimer J., Murphy C., Naylor J., Pearson M., Poon T.W.,
RA   Priest M., Roberts A., Saif S., Shea T., Sykes S., Wortman J., Nusbaum C.,
RA   Birren B.;
RT   "The genome sequence of Mycobacterium tuberculosis H37Rv.";
RL   Submitted (APR-2014) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC   -!- FUNCTION: Toxic component of a type II toxin-antitoxin (TA) system. An
CC       RNase. Its cognate antitoxin is VapB51. {ECO:0000305}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305};
CC   -!- SIMILARITY: Belongs to the PINc/VapC protein family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCP42957.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AL123456; CCP42957.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP003248; AFN48080.1; -; Genomic_DNA.
DR   EMBL; JLDD01000001; KBJ41338.1; -; Genomic_DNA.
DR   RefSeq; NP_214743.2; NC_000962.3.
DR   RefSeq; WP_003911071.1; NZ_NVQJ01000001.1.
DR   AlphaFoldDB; L0T5V6; -.
DR   SMR; L0T5V6; -.
DR   STRING; 83332.Rv0229c; -.
DR   PaxDb; L0T5V6; -.
DR   DNASU; 886724; -.
DR   GeneID; 45424200; -.
DR   GeneID; 886724; -.
DR   KEGG; mtu:Rv0229c; -.
DR   KEGG; mtv:RVBD_0229c; -.
DR   TubercuList; Rv0229c; -.
DR   eggNOG; COG1487; Bacteria.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR   HAMAP; MF_00265; VapC_Nob1; 1.
DR   InterPro; IPR029060; PIN-like_dom_sf.
DR   InterPro; IPR002716; PIN_dom.
DR   InterPro; IPR022907; VapC_family.
DR   Pfam; PF01850; PIN; 1.
DR   SUPFAM; SSF88723; SSF88723; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Magnesium; Metal-binding; Nuclease; Reference proteome;
KW   Toxin-antitoxin system.
FT   CHAIN           1..137
FT                   /note="Ribonuclease VapC51"
FT                   /id="PRO_0000430096"
FT   DOMAIN          5..120
FT                   /note="PINc"
FT   BINDING         8
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
FT   BINDING         95
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   137 AA;  15025 MW;  32452D3AFBCD3253 CRC64;
     MALKYLLDTS VIKRLSRPAV RRAVEPLAEA GAVARTQITD LEVGYSARNE TEWQRLMVAL
     SAFDLIESTA SHHRRALGIQ RLLAARSQRG RKIPDLLIAA AGEEHGLVVL HYDADFDLIA
     AVTGQPCQWI VPAGTID