VPH2_YEAST
ID VPH2_YEAST Reviewed; 215 AA.
AC P32341; D6VXH1;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Vacuolar ATPase assembly integral membrane protein VPH2;
DE AltName: Full=Protein VMA12;
GN Name=VPH2; Synonyms=CLS10, VMA12; OrderedLocusNames=YKL119C;
GN ORFNames=YKL520;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8419376; DOI=10.1016/s0021-9258(18)54027-8;
RA Hirata R., Umemoto N., Ho M.N., Ohya Y., Stevens T.H., Anraku Y.;
RT "VMA12 is essential for assembly of the vacuolar H(+)-ATPase subunits onto
RT the vacuolar membrane in Saccharomyces cerevisiae.";
RL J. Biol. Chem. 268:961-967(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8465604; DOI=10.1002/yea.320090208;
RA Bachhawat A.K., Manolson M.F., Murdock D.G., Garman J.D., Jones E.W.;
RT "The VPH2 gene encodes a 25 kDa protein required for activity of the yeast
RT vacuolar H(+)-ATPase.";
RL Yeast 9:175-184(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-73.
RX PubMed=1561835; DOI=10.1002/yea.320080207;
RA Jacquier A., Legrain P., Dujon B.;
RT "Sequence of a 10.7 kb segment of yeast chromosome XI identifies the APN1
RT and the BAF1 loci and reveals one tRNA gene and several new open reading
RT frames including homologs to RAD2 and kinases.";
RL Yeast 8:121-132(1992).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 72-215.
RX PubMed=1514329; DOI=10.1002/yea.320080410;
RA Colleaux L., Richard G.-F., Thierry A., Dujon B.;
RT "Sequence of a segment of yeast chromosome XI identifies a new
RT mitochondrial carrier, a new member of the G protein family, and a protein
RT with the PAAKK motif of the H1 histones.";
RL Yeast 8:325-336(1992).
RN [7]
RP SUBCELLULAR LOCATION, AND TOPOLOGY.
RX PubMed=9325326; DOI=10.1074/jbc.272.41.25928;
RA Jackson D.D., Stevens T.H.;
RT "VMA12 encodes a yeast endoplasmic reticulum protein required for vacuolar
RT H+-ATPase assembly.";
RL J. Biol. Chem. 272:25928-25934(1997).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Required for vacuolar ATPase assembly.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:9325326}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:9325326}.
CC -!- MISCELLANEOUS: Present with 2030 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; D11472; BAA02029.1; -; Genomic_DNA.
DR EMBL; M93350; AAA35212.1; -; Genomic_DNA.
DR EMBL; Z28118; CAA81960.1; -; Genomic_DNA.
DR EMBL; S93804; AAB22004.1; -; Genomic_DNA.
DR EMBL; S44213; AAB23070.2; -; Genomic_DNA.
DR EMBL; BK006944; DAA09041.1; -; Genomic_DNA.
DR PIR; S25358; S25358.
DR RefSeq; NP_012803.1; NM_001179685.1.
DR AlphaFoldDB; P32341; -.
DR SMR; P32341; -.
DR BioGRID; 34016; 228.
DR DIP; DIP-5907N; -.
DR IntAct; P32341; 4.
DR STRING; 4932.YKL119C; -.
DR TCDB; 9.B.206.2.1; the tmem199 (tmem199) family.
DR iPTMnet; P32341; -.
DR MaxQB; P32341; -.
DR PaxDb; P32341; -.
DR PRIDE; P32341; -.
DR DNASU; 853741; -.
DR EnsemblFungi; YKL119C_mRNA; YKL119C; YKL119C.
DR GeneID; 853741; -.
DR KEGG; sce:YKL119C; -.
DR SGD; S000001602; VPH2.
DR VEuPathDB; FungiDB:YKL119C; -.
DR eggNOG; ENOG502RZXR; Eukaryota.
DR HOGENOM; CLU_091774_0_0_1; -.
DR InParanoid; P32341; -.
DR OMA; WYWTGSS; -.
DR BioCyc; YEAST:G3O-31902-MON; -.
DR PRO; PR:P32341; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P32341; protein.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:1990871; C:Vma12-Vma22 assembly complex; IPI:SGD.
DR GO; GO:0007035; P:vacuolar acidification; IMP:SGD.
DR GO; GO:0070072; P:vacuolar proton-transporting V-type ATPase complex assembly; IMP:SGD.
DR InterPro; IPR021013; ATPase_Vma12.
DR PANTHER; PTHR31394; PTHR31394; 1.
DR Pfam; PF11712; Vma12; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..215
FT /note="Vacuolar ATPase assembly integral membrane protein
FT VPH2"
FT /id="PRO_0000065874"
FT TOPO_DOM 1..134
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 156..167
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..186
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..215
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 215 AA; 25287 MW; 96498A7AC1197C08 CRC64;
MFEIKLNDRI TEFLRKFKNS AKSNEGIDED IDLFLKRHAI PMQSLLFYVK EYRKDSDLQC
SIKELLKPLE FEFKPKAVRG LHYSEDFKKK LEFLKYQEQE LEYQSMVKRS KSVFSLQEDD
ELTPSQINKQ IKEQVTTVFN VLVSVISVVV AIWYWTGSST NFPVHVRLLL CLFFGILVLV
ADVVVYNSYL KKLEEAKVKE KTKVEKKKVL SKITL
