ID   VPH_STRVI               Reviewed;         287 AA.
AC   P18623;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=Viomycin phosphotransferase;
DE            EC=2.7.1.103;
DE   AltName: Full=Viomycin kinase;
GN   Name=vph;
OS   Streptomyces vinaceus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1960;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2987648; DOI=10.1007/bf00327505;
RA   Bibb M.J., Bibb M.J., Ward J.M., Cohen S.N.;
RT   "Nucleotide sequences encoding and promoting expression of three antibiotic
RT   resistance genes indigenous to Streptomyces.";
RL   Mol. Gen. Genet. 199:26-36(1985).
CC   -!- FUNCTION: The aminoglycoside phosphotransferases achieve inactivation
CC       of their antibiotic substrates by phosphorylation.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + viomycin = ADP + H(+) + O-phosphoviomycin;
CC         Xref=Rhea:RHEA:20509, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:60080, ChEBI:CHEBI:60081, ChEBI:CHEBI:456216;
CC         EC=2.7.1.103;
CC   -!- SIMILARITY: Belongs to the aminoglycoside phosphotransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X02393; CAA26235.1; -; Genomic_DNA.
DR   PIR; S28371; S28371.
DR   RefSeq; WP_030275963.1; NZ_JNYP01000012.1.
DR   AlphaFoldDB; P18623; -.
DR   SMR; P18623; -.
DR   GeneID; 63984161; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0050394; F:viomycin kinase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR002575; Aminoglycoside_PTrfase.
DR   InterPro; IPR011009; Kinase-like_dom_sf.
DR   Pfam; PF01636; APH; 1.
DR   SUPFAM; SSF56112; SSF56112; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; ATP-binding; Kinase; Nucleotide-binding;
KW   Transferase.
FT   CHAIN           1..287
FT                   /note="Viomycin phosphotransferase"
FT                   /id="PRO_0000204817"
FT   ACT_SITE        190
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   287 AA;  30468 MW;  A621022B91E13D29 CRC64;
     MRIIETHRDL LSRLLPGDTV GGLAVHEGQF HHVVIGSHRV VCFARTRAAA DRLPGRADVL
     RALAGIDLGF RTPQPLSEGG AQGTDEPPYL VLSRIPGAPL EDDVLTSPEV AEAVARQYAT
     LLSGLAAAGD EEKVRAALPE APANEWQEFA TGVRTELFPL MSDGGRERAE RELAALDALP
     HLTSAVVHGD LGGENVLWET VDGVPRMSGV VDWDEVGIGD PAEDLAAIGA SYGEELLGRV
     LALGGWADNG TAERISAIRG TFALQQALYA QRDGDEEELA DGLSGYR