ID   VPI1_SCOJE              Reviewed;          94 AA.
AC   P0DM55;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2013, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Venom peptide SjAPI {ECO:0000303|PubMed:23533574};
DE   AltName: Full=Ascaris-type protease inhibitor {ECO:0000305};
DE   Flags: Precursor;
OS   Scorpiops jendeki (Scorpion) (Scorpiops hardwickii jendeki).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida;
OC   Scorpiones; Iurida; Chactoidea; Euscorpiidae; Scorpiopinae; Scorpiopini;
OC   Scorpiops.
OX   NCBI_TaxID=587368;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF 63-ALA--VAL-65,
RP   CIRCULAR DICHROISM, AND 3D-STRUCTURE MODELING.
RC   TISSUE=Venom gland;
RX   PubMed=23533574; DOI=10.1371/journal.pone.0057529;
RA   Chen Z., Wang B., Hu J., Yang W., Cao Z., Zhuo R., Li W., Wu Y.;
RT   "SjAPI, the first functionally characterized Ascaris-type protease
RT   inhibitor from animal venoms.";
RL   PLoS ONE 8:E57529-E57529(2013).
CC   -!- FUNCTION: Recombinant protein inhibits both alpha-chymotrypsin (Ki=97.1
CC       nM) and elastase (Ki=3700 nM). {ECO:0000269|PubMed:23533574}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:23533574}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:23533574}.
CC   -!- MISCELLANEOUS: Does not inhibit trypsin. {ECO:0000305|PubMed:23533574}.
CC   -!- SIMILARITY: Belongs to the serine protease inhibitor-like (TIL domain-
CC       containing) family. {ECO:0000305}.
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DR   AlphaFoldDB; P0DM55; -.
DR   SMR; P0DM55; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR   InterPro; IPR036084; Ser_inhib-like_sf.
DR   InterPro; IPR002919; TIL_dom.
DR   Pfam; PF01826; TIL; 1.
DR   SUPFAM; SSF57567; SSF57567; 1.
PE   2: Evidence at transcript level;
KW   Disulfide bond; Protease inhibitor; Secreted; Serine protease inhibitor;
KW   Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   PROPEP          25..30
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000423041"
FT   CHAIN           31..94
FT                   /note="Venom peptide SjAPI"
FT                   /id="PRO_0000423042"
FT   DOMAIN          33..92
FT                   /note="TIL"
FT   REGION          63..65
FT                   /note="Protease binding loop"
FT   DISULFID        33..70
FT                   /evidence="ECO:0000250"
FT   DISULFID        43..66
FT                   /evidence="ECO:0000250"
FT   DISULFID        47..62
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..92
FT                   /evidence="ECO:0000250"
FT   DISULFID        72..86
FT                   /evidence="ECO:0000250"
FT   MUTAGEN         63..65
FT                   /note="AAV->PLM: Inhibits both chymotrypsin and elastase,
FT                   but not trypsin."
FT                   /evidence="ECO:0000269|PubMed:23533574"
FT   MUTAGEN         63..65
FT                   /note="AAV->TKD: Inhibits both chymotrypsin and elastase,
FT                   but not trypsin."
FT                   /evidence="ECO:0000269|PubMed:23533574"
FT   MUTAGEN         63..65
FT                   /note="AAV->TLE: Inhibits both trypsin and chymotrypsin,
FT                   but not elastase."
FT                   /evidence="ECO:0000269|PubMed:23533574"
FT   MUTAGEN         63..65
FT                   /note="AAV->TME: Inhibits both chymotrypsin and elastase,
FT                   but not trypsin."
FT                   /evidence="ECO:0000269|PubMed:23533574"
FT   MUTAGEN         63..65
FT                   /note="AAV->TMQ: Inhibits both chymotrypsin and elastase,
FT                   but not trypsin."
FT                   /evidence="ECO:0000269|PubMed:23533574"
FT   MUTAGEN         63..65
FT                   /note="AAV->TRE: Inhibits both trypsin and chymotrypsin,
FT                   but not elastase."
FT                   /evidence="ECO:0000269|PubMed:23533574"
SQ   SEQUENCE   94 AA;  10312 MW;  72BD4FE165B0761F CRC64;
     MKWGALLCIF GFLAFCSVLD RGLGWIPDIW QKCSSKNEEF QQCGSSCPET CANHKNPEPK
     SCAAVCFVGC VCKPGFIRDD LKGSICVKPE DCSK