CALRL_MOUSE
ID CALRL_MOUSE Reviewed; 463 AA.
AC Q9R1W5; Q9QXH8; Q9WUP2;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 26-SEP-2003, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Calcitonin gene-related peptide type 1 receptor;
DE Short=CGRP type 1 receptor;
DE AltName: Full=Calcitonin receptor-like receptor;
DE Flags: Precursor;
GN Name=Calcrl; Synonyms=Crlr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC TISSUE=Spleen;
RX PubMed=12147211; DOI=10.1054/npep.2002.0871;
RA Miyauchi K., Tadotsu N., Hayashi T., Ono Y., Tokoyoda K., Tsujikawa K.,
RA Yamamoto H.;
RT "Molecular cloning and characterization of mouse calcitonin gene-related
RT peptide receptor.";
RL Neuropeptides 36:22-33(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Derst C., Preisig-Mueller R., Gerhardus J., Daut J.;
RT "Cloning and sequencing of mouse CGRP/adrenomedullin receptor subunits.";
RL Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J; TISSUE=Lung;
RX PubMed=10777702; DOI=10.1006/bbrc.2000.2606;
RA Ono Y., Okano I., Kojima M., Okada K., Kangawa K.;
RT "Decreased gene expression of adrenomedullin receptor in mouse lungs during
RT sepsis.";
RL Biochem. Biophys. Res. Commun. 271:197-202(2000).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-419 AND SER-444, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Receptor for calcitonin-gene-related peptide (CGRP) together
CC with RAMP1 and receptor for adrenomedullin together with RAMP2 or
CC RAMP3. The activity of this receptor is mediated by G proteins which
CC activate adenylyl cyclase (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of CALCRL and RAMP3 (By similarity). Heterodimer
CC of CALCRL and RAMP1 or CALCRL and RAMP2 (By similarity).
CC {ECO:0000250|UniProtKB:Q16602}.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in the lung, thymus, heart
CC and brain. {ECO:0000269|PubMed:10777702, ECO:0000269|PubMed:12147211}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family.
CC {ECO:0000305}.
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DR EMBL; AB015595; BAA76492.1; -; mRNA.
DR EMBL; AF146525; AAD35021.1; -; mRNA.
DR EMBL; AF209905; AAF21037.1; -; mRNA.
DR CCDS; CCDS16184.1; -.
DR RefSeq; NP_061252.2; NM_018782.2.
DR RefSeq; XP_006499981.1; XM_006499918.3.
DR AlphaFoldDB; Q9R1W5; -.
DR SMR; Q9R1W5; -.
DR ComplexPortal; CPX-3149; CGRP receptor complex.
DR ComplexPortal; CPX-3150; Adrenomedullin receptor AM1 complex.
DR ComplexPortal; CPX-3151; Adrenomedullin receptor AM2 complex.
DR STRING; 10090.ENSMUSP00000073875; -.
DR BindingDB; Q9R1W5; -.
DR ChEMBL; CHEMBL2034811; -.
DR GuidetoPHARMACOLOGY; 47; -.
DR GlyGen; Q9R1W5; 6 sites.
DR iPTMnet; Q9R1W5; -.
DR PhosphoSitePlus; Q9R1W5; -.
DR MaxQB; Q9R1W5; -.
DR PaxDb; Q9R1W5; -.
DR PRIDE; Q9R1W5; -.
DR ProteomicsDB; 265511; -.
DR Antibodypedia; 1959; 240 antibodies from 32 providers.
DR DNASU; 54598; -.
DR Ensembl; ENSMUST00000074262; ENSMUSP00000073875; ENSMUSG00000059588.
DR Ensembl; ENSMUST00000099944; ENSMUSP00000097527; ENSMUSG00000059588.
DR GeneID; 54598; -.
DR KEGG; mmu:54598; -.
DR UCSC; uc008kii.3; mouse.
DR CTD; 10203; -.
DR MGI; MGI:1926944; Calcrl.
DR VEuPathDB; HostDB:ENSMUSG00000059588; -.
DR eggNOG; KOG4564; Eukaryota.
DR GeneTree; ENSGT00940000159898; -.
DR HOGENOM; CLU_002753_4_2_1; -.
DR InParanoid; Q9R1W5; -.
DR OMA; CVALHII; -.
DR OrthoDB; 1005634at2759; -.
DR PhylomeDB; Q9R1W5; -.
DR TreeFam; TF315710; -.
DR Reactome; R-MMU-418555; G alpha (s) signalling events.
DR Reactome; R-MMU-419812; Calcitonin-like ligand receptors.
DR BioGRID-ORCS; 54598; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Calcrl; mouse.
DR PRO; PR:Q9R1W5; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9R1W5; protein.
DR Bgee; ENSMUSG00000059588; Expressed in left lung lobe and 228 other tissues.
DR ExpressionAtlas; Q9R1W5; baseline and differential.
DR Genevisible; Q9R1W5; MM.
DR GO; GO:1903143; C:adrenomedullin receptor complex; ISO:MGI.
DR GO; GO:1990406; C:CGRP receptor complex; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005783; C:endoplasmic reticulum; ISO:MGI.
DR GO; GO:0005768; C:endosome; ISO:MGI.
DR GO; GO:0005764; C:lysosome; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:1990409; F:adrenomedullin binding; ISO:MGI.
DR GO; GO:0001605; F:adrenomedullin receptor activity; ISO:MGI.
DR GO; GO:0001635; F:calcitonin gene-related peptide receptor activity; IGI:MGI.
DR GO; GO:0004948; F:calcitonin receptor activity; IEA:InterPro.
DR GO; GO:0008528; F:G protein-coupled peptide receptor activity; IBA:GO_Central.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IMP:MGI.
DR GO; GO:0007188; P:adenylate cyclase-modulating G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:1990410; P:adrenomedullin receptor signaling pathway; ISO:MGI.
DR GO; GO:0001525; P:angiogenesis; ISO:MGI.
DR GO; GO:1990408; P:calcitonin gene-related peptide receptor signaling pathway; ISO:MGI.
DR GO; GO:0006816; P:calcium ion transport; ISO:MGI.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR GO; GO:0071329; P:cellular response to sucrose stimulus; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; TAS:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0050728; P:negative regulation of inflammatory response; ISO:MGI.
DR GO; GO:0045986; P:negative regulation of smooth muscle contraction; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:MGI.
DR GO; GO:1904707; P:positive regulation of vascular associated smooth muscle cell proliferation; IMP:MGI.
DR GO; GO:0015031; P:protein transport; ISO:MGI.
DR GO; GO:0031623; P:receptor internalization; ISO:MGI.
DR GO; GO:0048659; P:smooth muscle cell proliferation; IMP:MGI.
DR GO; GO:1990874; P:vascular associated smooth muscle cell proliferation; IMP:MGI.
DR Gene3D; 4.10.1240.10; -; 1.
DR InterPro; IPR003287; GCPR_2_calcitonin_rcpt_fam.
DR InterPro; IPR017981; GPCR_2-like.
DR InterPro; IPR003289; GPCR_2_CGRP1_rcpt.
DR InterPro; IPR036445; GPCR_2_extracell_dom_sf.
DR InterPro; IPR001879; GPCR_2_extracellular_dom.
DR InterPro; IPR000832; GPCR_2_secretin-like.
DR InterPro; IPR017983; GPCR_2_secretin-like_CS.
DR Pfam; PF00002; 7tm_2; 1.
DR Pfam; PF02793; HRM; 1.
DR PRINTS; PR01351; CGRPRECEPTOR.
DR PRINTS; PR01350; CTRFAMILY.
DR PRINTS; PR00249; GPCRSECRETIN.
DR SMART; SM00008; HormR; 1.
DR SUPFAM; SSF111418; SSF111418; 1.
DR PROSITE; PS00649; G_PROTEIN_RECEP_F2_1; 1.
DR PROSITE; PS00650; G_PROTEIN_RECEP_F2_2; 1.
DR PROSITE; PS50227; G_PROTEIN_RECEP_F2_3; 1.
DR PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Signal; Transducer;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..463
FT /note="Calcitonin gene-related peptide type 1 receptor"
FT /id="PRO_0000012812"
FT TOPO_DOM 23..145
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 146..165
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 166..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..192
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..235
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..252
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 253..272
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..288
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..312
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..335
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..353
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 354..365
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..387
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..463
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 419
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 444
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CARBOHYD 29
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 65
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 117
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 128
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 47..73
FT /evidence="ECO:0000250"
FT DISULFID 64..104
FT /evidence="ECO:0000250"
FT DISULFID 87..126
FT /evidence="ECO:0000250"
FT CONFLICT 235
FT /note="Y -> C (in Ref. 1; BAA76492)"
FT /evidence="ECO:0000305"
FT CONFLICT 458
FT /note="M -> T (in Ref. 2; AAD35021)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 463 AA; 53265 MW; 630EC8956A4FB47E CRC64;
MDKKHILCFL VLLPLNMALI SAESEEGVNQ TDLGVTRNKI MTAQYECYQK IMQDPIQQAE
GLYCNRTWDG WLCWNDVAAG TESMQYCPDY FQDFDPSEKV TKICDQDGHW FRHPDSNRTW
TNYTLCNNST HEKVKTALNL FYLTIIGHGL SIASLIISLI IFFYFKSLSC QRITLHKNLF
FSFICNSIVT IIHLTAVANN QALVATNPVS CKVSQFIHLY LMGCNYFWML CEGVYLHTLI
VVAVFAEKQH LMWYYFLGWG FPLLPACIHA IARSLYYNDN CWISSDTHLL YIIHGPICAA
LLVNLFFLLN IVRVLITKLK VTHQVESNLY MKAVRATLIL VPLLGIEFVL FPWRPEGKVA
EEVYDYVMHI LMHFQGLLVA TIFCFFNGEV QAILRRNWNQ YKIQFGNGFS HSDALRSASY
TVSTISDMQG YSHDCPTEHL NGKSIQDIEN VALKSENMYD LVM
