ID   VPS10_AJECN             Reviewed;        1477 AA.
AC   A6QWZ2;
DT   03-MAY-2011, integrated into UniProtKB/Swiss-Prot.
DT   21-AUG-2007, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Vacuolar protein sorting/targeting protein 10;
DE   AltName: Full=Carboxypeptidase Y receptor;
DE            Short=CPY receptor;
DE   AltName: Full=Sortilin VPS10;
DE   AltName: Full=Vacuolar carboxypeptidase sorting receptor VPS10;
DE   Flags: Precursor;
GN   Name=VPS10; ORFNames=HCAG_01899;
OS   Ajellomyces capsulatus (strain NAm1 / WU24) (Darling's disease fungus)
OS   (Histoplasma capsulatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Onygenales; Ajellomycetaceae; Histoplasma;
OC   unclassified Histoplasma.
OX   NCBI_TaxID=2059318;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NAm1 / WU24;
RX   PubMed=19717792; DOI=10.1101/gr.087551.108;
RA   Sharpton T.J., Stajich J.E., Rounsley S.D., Gardner M.J., Wortman J.R.,
RA   Jordar V.S., Maiti R., Kodira C.D., Neafsey D.E., Zeng Q., Hung C.-Y.,
RA   McMahan C., Muszewska A., Grynberg M., Mandel M.A., Kellner E.M.,
RA   Barker B.M., Galgiani J.N., Orbach M.J., Kirkland T.N., Cole G.T.,
RA   Henn M.R., Birren B.W., Taylor J.W.;
RT   "Comparative genomic analyses of the human fungal pathogens Coccidioides
RT   and their relatives.";
RL   Genome Res. 19:1722-1731(2009).
CC   -!- FUNCTION: Functions as a sorting receptor in the Golgi compartment
CC       required for the intracellular sorting and delivery of soluble vacuolar
CC       proteins, like carboxypeptidase Y (CPY) and proteinase A. Executes
CC       multiple rounds of sorting by cycling between the late Golgi and a
CC       prevacuolar endosome-like compartment (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus, trans-Golgi network membrane
CC       {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}. Prevacuolar
CC       compartment membrane {ECO:0000250}; Multi-pass membrane protein
CC       {ECO:0000250}. Note=Cycles between the Golgi apparatus and the
CC       prevacuolar compartment. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the VPS10 family. {ECO:0000305}.
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DR   EMBL; CH476655; EDN04034.1; -; Genomic_DNA.
DR   RefSeq; XP_001544852.1; XM_001544802.1.
DR   AlphaFoldDB; A6QWZ2; -.
DR   SMR; A6QWZ2; -.
DR   STRING; 339724.A6QWZ2; -.
DR   EnsemblFungi; EDN04034; EDN04034; HCAG_01899.
DR   GeneID; 5449880; -.
DR   KEGG; aje:HCAG_01899; -.
DR   VEuPathDB; FungiDB:HCAG_01899; -.
DR   HOGENOM; CLU_000700_0_0_1; -.
DR   OMA; ATMSEFI; -.
DR   OrthoDB; 1046610at2759; -.
DR   Proteomes; UP000009297; Unassembled WGS sequence.
DR   GO; GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR031777; Sortilin_C.
DR   InterPro; IPR031778; Sortilin_N.
DR   InterPro; IPR006581; VPS10.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   Pfam; PF15902; Sortilin-Vps10; 2.
DR   Pfam; PF15901; Sortilin_C; 2.
DR   SMART; SM00602; VPS10; 2.
PE   3: Inferred from homology;
KW   Glycoprotein; Golgi apparatus; Membrane; Protein transport; Receptor;
KW   Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW   Transport.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..1477
FT                   /note="Vacuolar protein sorting/targeting protein 10"
FT                   /id="PRO_0000407496"
FT   TOPO_DOM        22..1325
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1326..1346
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1347..1372
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1373..1393
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        1394..1477
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   REPEAT          61..71
FT                   /note="BNR 1"
FT   REPEAT          351..360
FT                   /note="BNR 2"
FT   REPEAT          411..421
FT                   /note="BNR 3"
FT   REPEAT          453..463
FT                   /note="BNR 4"
FT   REPEAT          695..705
FT                   /note="BNR 5"
FT   REPEAT          1079..1089
FT                   /note="BNR 6"
FT   REPEAT          1121..1130
FT                   /note="BNR 7"
FT   REGION          1443..1463
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        295
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        944
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1201
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1216
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        1237
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1477 AA;  165599 MW;  C831C47BA7BC3CF4 CRC64;
     MILRRLLLAG SLLLATSFTS AKKADGPKIS ATKFKDEPVN LFYFDDSDTV MFQDGKNGDV
     YVSRDAGANW DIVDGGTHWV TEDQAKSWRE FTVDAELSRY EYPLVFHGKD SNRVMLLGHK
     CKGLDCKERT YYTTDGFKTV HLLMENGRHC AWAVSTPTFG EGLDLPKEVN DRIFCVVSGL
     HSSWAEANRL LYSDRFFKDE QGTEVPLDNG RAVSGVIRTA SVQKYILAAT KSARTNELAL
     FVTDDASTWH RTEFDGHRVE EDAYTILEST SYSLQVDVVS TYSSTIGTLF TSNSNGTYFT
     RNIKHTNRNL YGFVDFEKIS SIQGIILVNT VKNWEDVEKS NGVQKKVISK ISFDDGRTFQ
     PLKVGKHDLH LHSVTDATNS GRVFSSPAPG LVMGVGNTGG HLKDYLDGDL FVSDDAGINW
     RKALDDAHKY EFGDQGSVIV AVFDEGRTDK ISYSLDHGKN WHKASLPDGV QIRAKVLTTA
     PGSTTLKFLL LGSAKADIGM EYYIISIDFA EMEERTCEEK DFENWPARLN EKNEPDCLMG
     HKQFYRRRKA QADCFIKKKF EEPAPEFERC KCTEEDFECD FNFVRGEDGK SCIPSRSLIA
     PEGVCKNPDD KFIGSSGFRL IPGNVCIREG GVDLDKQTER VCSETFKNPP AGQIVVEKSF
     FTADYYKDYF YLERKESSKG EDETVIMITS EQHIFLSRDH GKKWKRILEE EKITRIEPHR
     FFDDVAYFLT NNGDGWYTLD RGDTFRKFKA PLPPNQDKLP VLSFHPDRRD WLIWTGADEC
     NGNGGNCHSV AYYTTNLGGE WHFLMRYVRR CEFISRDARG SSDKLVFCEQ FENENPSNKH
     LQLLSTEDWF AEKRLHYSNI LDFATMQEFI IVAIRGENPQ DSLRIGVSID GKTFADAELP
     ANVQIPIQRA YTVLESRTHA AFLLVTINNM EDHEYGSILK SNSNGTSYVL SLSAVNRNSR
     GYADFEKMQG LEGVAMANVV GNVADVENGA AKKFRTMITH NDGAEWTLVR PPDKDSEGRS
     YACSTKGGKP TDACALHLHS YTERADPRDT YSSPSAVGIM IGTGNVGDYL SLKSKADTFI
     TRDAGITWEE VKKGKYQWEF GDSGSIIVLV TESTPTKTLL YSLDEGRSWK DFEFSEVEMQ
     IQDISTVPSD TSRNFLLWGK EVGQGKKPGL ATVNIDFSGL KERSKHIPFN LMDASSVTEP
     NITANGPTRI AITAENYSTW TPLGISANAH DRIMNGNKTD LLICSDYNYE PQSDGSCARV
     AGLEPLDPKL VCTENPKAIE WYEPTGYRRI PLTKCQGGKQ LNHIVAHPCP NKEEEFFKKH
     PRLRGIGLFF VILIPICLAA TSGYYVYNHW DGKFGRIRLG ETGSGGLFDR DSLLVSIPVS
     MVAGVVAVIT ALPLLVSSLW RSVSGYVRVP GGASSRRPYS SRDSFAARRN DYTGVVEDED
     ELLGTDDFDD DEEGDERNGQ MKNNGHVNEA ITIVFLF